1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. COX-2 蛋白, Human (sf9, His)

COX-2 蛋白, Human (sf9, His)

目录号: HY-P74222
COA 产品使用指南

COX-2蛋白参与前列腺素生物合成的替代途径。COX-2 蛋白, Human (sf9, His) 是重组的 COX-2 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。COX-2 蛋白, Human (sf9, His) 全长 604 个氨基酸,分子量约为 ~66 kDa。

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规格 价格 是否有货 数量
50 μg ¥4750 In-stock
100 μg   询价  

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描述

COX-2 protein is involved in the alternative pathway of prostaglandin biosynthesis. COX-2 Protein, Human (sf9, His) is the recombinant human-derived COX-2 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of COX-2 Protein, Human (sf9, His) is 604 a.a., with molecular weight of ~66 kDa.

研究背景

COX-2 蛋白在前列腺素生物合成途径中充当双重功能酶,前列腺素是炎症反应的关键介质。COX-2 同时充当环氧合酶和过氧化物酶,将花生四烯酸 (AA) 转化为氢过氧内过氧化物前列腺素 G2 (PGG2),并进一步将其还原为羟基内过氧化物前列腺素 H2 (PGH2)。该催化过程涉及碳 13 处的氢提取,然后插入分子氧以形成前列腺素中特有的内过氧化物桥。COX-2 将其酶活性扩展到二高γ-亚油酸 (DGLA) 和二十碳五烯酸 (EPA),生成 PGH1 和 PGH3 作为 1 系列和 3 系列前列腺素的前体。此外,COX-2 有助于替代前列腺素生物合成途径,转化 2-花生四烯酰溶血磷脂并代谢 2-花生四烯酰甘油。该酶还在分解相相互作用产物的生成中发挥作用,有助于在炎症过程中合成专门的促分解介质,例如分解素。这种多方面的功能凸显了 COX-2 在前列腺素生成和炎症信号传导的复杂调节中的关键作用。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P35354 (M1-L604)

基因 ID
蛋白结构
N-term
COX-2 (M1-L604)
Accession # P35354
His
C-term
同用名
Prostaglandin G/H synthase 2; COX-2; PGHS-2
分子量

Approximately 66 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

COX-2 Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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COX-2 Protein, Human (sf9, His)
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