1. 重组蛋白
  2. Enzymes & Regulators
  3. Carboxypeptidase
  4. Carboxypeptidase A
  5. Carboxypeptidase A2
  6. CPA2 蛋白, Mouse (HEK293, His)

CPA2 蛋白, Mouse (HEK293, His)

目录号: HY-P70072
产品使用指南

CPA2 蛋白是编码锌金属蛋白酶羧基肽酶 A 家族的一个成员, 由胰腺外分泌细胞表达。CPA2 蛋白 作用于芳香 C 端残基,在降解食物形成氨基酸过程中起重要作用。CPA2 蛋白, Mouse (HEK293, His) 是重组的 CPA2 蛋白,由 HEK293 表达,带有 C-6*His 标签。CPA2 蛋白, Mouse (HEK293, His) 全长 401 个氨基酸,分子量为 41-50 kDa。

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描述

CPA2 Protein is A member of the carboxypeptidase family that encodes zinc metalloproteinases and is expressed by pancreatic exocrine cells. CPA2 Protein acts on aromatic C-terminal residues and plays an important role in the process of degrading food to form amino acids. CPA2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CPA2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CPA2 Protein, Mouse (HEK293, His) is 401 a.a., with molecular weight of 41-50 kDa.

研究背景

Carboxypeptidase A2 (CPA2) 是编码锌金属蛋白酶羧基肽酶 A 家族的一个成员。编码的前原蛋白经过蛋白水解处理,去除 N 端激活肽,生成功能性酶。目前已分离出三种不同形式的人胰腺原羧肽酶 A ,A1 和 A2 是具有不同生化特性的单体蛋白。A2 形式的胰腺原羧肽酶作用于芳香 C 端残基。底物抑制对芳香 N-酰基二肽底物及其酯类似物的抑制明显CPA2 由胰腺外分泌细胞表达,在消化过程中分泌这种酶。该基因位于 6 号染色体上的羧基肽酶基因簇中。CPA2 在降解食物蛋白质导致氨基酸形成的过程中很重要。CPA2 在肾素-血管紧张素系统中也可发挥作用。

种属

Mouse

表达系统

HEK293

标签

C-6*His

蛋白编号

Q504N0 (Q17-Y417)

基因 ID

232680  [NCBI]

蛋白结构
N-term
CPA2 (Q17-Y417)
Accession # Q504N0
6*His
C-term
同用名
rMuCarboxypeptidase A2/CPA2, His; CPA2; Carboxypeptidase A2
氨基酸序列

QETFVGDQVLEVIPNDEEQIKTLLQLEAEEHLELDFWKSPSVPRQTVHVRVPFASIQDVKVFLESQGITYSIMIEDVQVLLDQEREEMLFNQQRERGTNFNFGAYHTLEEIYQEMDNLVAENPGLVSKVNIGSSFENRPMNVLKFSTGGDKPAIWLDAGIHAREWVTQATALWTANKIASDYGTDPAITSLLNTLDVFLLPVTNPDGYVFSQTSNRMWRKTRSKRSGSFCVGVDPNRNWDANFGGPGASSNPCSDSYHGPSPNSEVEVKSIVDFIKSHGKVKAFITLHSYSQLLMFPYGYKCAKPDDFNELDEVAQRAAQSLKRLHGTSYKVGPICSVIYQASGGSIDWAYDLGIKYSFAFELRDTGYYGFLLPAKQILPTAEETWLGLKTIMEHVRDHPY

分子量

41-50 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CPA2 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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