2. 重组蛋白
  3. Enzymes & Regulators
  4. Transferases (EC 2)
  5. Creatine kinase M-type/CKM 蛋白, Mouse (His)

Creatine kinase M-type/CKM 蛋白, Mouse (His)

目录号: HY-P700577
产品使用指南

CKM 是一种肌酸激酶 M 型蛋白，集中催化 ATP 和各种磷原（尤其是磷酸肌酸）之间的可逆磷酸盐转移。作为肌酸激酶同工酶，CKM 在能量转导中发挥着至关重要的作用，特别是在能量需求较大且波动的组织中，例如骨骼肌、心脏、大脑和精子。Creatine kinase M-type/CKM 蛋白, Mouse (His) 是重组的 肌酸激酶 M 型/CKM 蛋白，由 E. coli 表达，带有 N-6*His 标签。Creatine kinase M-type/CKM 蛋白, Mouse (His) 全长 381 个氨基酸，分子量为 49.0 kDa。

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描述

CKM is a creatine kinase M-type protein that centrally catalyzes reversible phosphate transfer between ATP and various phosphogens, especially creatine phosphate. As a creatine kinase isoenzyme, CKM plays a crucial role in energy transduction, particularly in tissues with large and fluctuating energy demands, such as skeletal muscle, heart, brain, and sperm. Creatine kinase M-type/CKM Protein, Mouse (His) is the recombinant mouse-derived Creatine kinase M-type/CKM protein, expressed by E. coli , with N-6*His labeled tag. The total length of Creatine kinase M-type/CKM Protein, Mouse (His) is 381 a.a., with molecular weight of 49.0 kDa.
研究背景

肌酸激酶 M 型 (CKM) 蛋白对于可逆催化 ATP 和各种磷原（包括磷酸肌酸）之间的磷酸盐转移至关重要。作为肌酸激酶同工酶，CKM 在能量转导过程中发挥着核心作用，特别是在能量需求大量且波动的组织中。这些组织包括骨骼肌、心脏、大脑和精子，CKM 促进能量的有效利用和储存。在此过程中，以 CKM 为代表的肌酸激酶同工酶对维持能量稳态和满足这些重要组织内的动态代谢需求做出了重大贡献。
种属

Mouse
表达系统

E. coli
标签

N-6*His
蛋白编号

P07310 (M1-K381)
基因 ID

12715  [NCBI]
蛋白结构
N-term
6*His
CKM (M1-K381)
Accession # P07310
C-term
同用名
rHuCreatine kinase M-type/CKMM, His; Creatine kinase M-type; Creatine kinase M chain; M-CK; CKM; CKMM
氨基酸序列

MPFGNTHNKFKLNYKPQEEYPDLSKHNNHMAKVLTPDLYNKLRDKETPSGFTLDDVIQTGVDNPGHPFIMTVGCVAGDEESYTVFKDLFDPIIQDRHGGYKPTDKHKTDLNHENLKGGDDLDPNYVLSSRVRTGRSIKGYTLPPHCSRGERRAVEKLSVEALNSLTGEFKGKYYPLKSMTEQEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKSFLVWVNEEDHLRVISMEKGGNMKEVFRRFCVGLQKIEEIFKKAGHPFMWNEHLGYVLTCPSNLGTGLRGGVHVKLANLSKHPKFEEILTRLRLQKRGTGGVDTAAVGAVFDISNADRLGSSEVEQVQLVVDGVKLMVEMEKKLEKGQSIDDMIPAQK

分子量

49.0 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Creatine kinase M-type/CKM Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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