CRMP1 蛋白, Human

The CRMP1 protein is indispensable for class 3 semaphorin-mediated signaling that drives cytoskeletal remodeling critical for axonal guidance. CRMP1 downstream of SEMA3A induces FLNA dissociation from F-actin, promoting actin cytoskeleton reorganization and growth cone collapse. CRMP1 Protein, Human is the recombinant human-derived CRMP1 protein, expressed by E. coli , with tag free. The total length of CRMP1 Protein, Human is 572 a.a., with molecular weight of ~62.2 kDa.

CRMP1 蛋白对于 3 类信号蛋白介导的信号传导至关重要，在随后的细胞骨架重塑中发挥着至关重要的作用。它积极参与轴突引导，作用于 SEMA3A 下游，诱导 FLNA 从 F-肌动蛋白解离，导致肌动蛋白细胞骨架重组以及随后生长锥的塌陷。除了在轴突引导中的作用之外，CRMP1 还参与侵袭性生长和细胞迁移过程，表明它参与细胞动力学。此外，有证据表明其对胞质分裂的潜在贡献。CRMP1 与 DPYSL2、DPYSL3、DPYSL4 或 DPYSL5 形成同源四聚体和异源四聚体，表明其与相关蛋白的关联。此外，它与 PLXNA1 和 FLNA 相互作用，特别是通过 FLNA 的钙调蛋白同源 (CH) 结构域 1 和细丝重复序列 24，改变 FLNA 的三元结构并促进其与 F-肌动蛋白的解离，突出其在细胞骨架动力学中的复杂参与。

Human

E. coli

Tag Free

Q14194 (M1-G572)

1400  [NCBI]

MSYQGKKSIPHITSDRLLIKGGRIINDDQSLYADVYLEDGLIKQIGENLIVPGGVKTIEANGRMVIPGGIDVNTYLQKPSQGMTAADDFFQGTRAALVGGTTMIIDHVVPEPGSSLLTSFEKWHEAADTKSCCDYSLHVDITSWYDGVREELEVLVQDKGVNSFQVYMAYKDVYQMSDSQLYEAFTFLKGLGAVILVHAENGDLIAQEQKRILEMGITGPEGHALSRPEELEAEAVFRAITIAGRINCPVYITKVMSKSAADIIALARKKGPLVFGEPIAASLGTDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYLTSLLACGDLQVTGSGHCPYSTAQKAVGKDNFTLIPEGVNGIEERMTVVWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRIAVGSDADVVIWDPDKLKTITAKSHKSAVEYNIFEGMECHGSPLVVISQGKIVFEDGNINVNKGMGRFIPRKAFPEHLYQRVKIRNKVFGLQGVSRGMYDGPVYEVPATPKYATPAPSAKSSPSKHQPPPIRNLHQSNFSLSGAQIDDNNPRRTGHRIVAPPGGRSNITSLG

Approximately 62.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.