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  4. CS6 fimbrial subunit B/cssB 蛋白, E.coli (His-SUMO)

CS6 fimbrial subunit B/cssB 蛋白, E.coli (His-SUMO)

目录号: HY-P71473
产品使用指南

CS6 菌毛亚基 B (cssB) 是大肠杆菌 CS6 操纵子的一个组成部分。CssB 是与细胞表面硫苷脂结合的结构亚基,是与宿主细胞结合的关键因子。CssB 也是稳定 CssA、大肠杆菌与上皮细胞的最大粘附和 CS6 组装所必需的。CS6 fimbrial subunit B/cssB 蛋白, E.coli (His-SUMO) 是重组的 CS6 菌毛亚基 B/cssB 蛋白,由 E. coli 表达,带有 N-His, N-SUMO 标签。CS6 fimbrial subunit B/cssB 蛋白, E.coli (His-SUMO) 全长 146 个氨基酸,分子量约为 ~31.9 kDa。

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描述

CS6 fimbrial subunit B (cssB) is a component of E.coli CS6 operon. CssB is a structural subunit which binds to cell surface sulfatide and is a key factor for binding to host cells. CssB is also required for stabilization of CssA, maximal adhesion of E.coli to epithelial cells and CS6 assembly. CS6 fimbrial subunit B/cssB Protein, E.coli (His-SUMO) is the recombinant E. coli-derived CS6 fimbrial subunit B/cssB protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of CS6 fimbrial subunit B/cssB Protein, E.coli (His-SUMO) is 146 a.a., with molecular weight of ~31.9 kDa.

研究背景

CS6 菌毛亚基 B (cssB) 是大肠杆菌 CS6 操纵子的一个组成部分。CssB 是与细胞表面硫苷脂结合的结构亚基,是与宿主细胞结合的关键因子。CssA 作为 CS6 的另一个结构亚基,可以抑制 CssB 介导的结合。CssB 也是稳定 CssA 所必需的,而所有 css 基因对于大肠杆菌与上皮细胞的最大粘附和 CS6 组装都是必需的[1]

种属

E.coli

表达系统

E. coli

标签

N-His;N-SUMO

蛋白编号

P53510 (22G-167N)

基因 ID

/

蛋白结构
N-term
6*His-SUMO
cssB (22G-167N)
Accession # P53510
C-term
同用名
cssBCS6 fimbrial subunit B
氨基酸序列

GNWQYKSLDVNVNIEQNFIPDIDSAVRIIPVNYDSDPKLNSQLYTVEMTIPAGVSAVKIVPTDSLTSSGQQIGKLVNVNNPDQNMNYYIRKDSGAGKFMAGQKGSFSVKENTSYTFSAIYTGGEYPNSGYSSGTYAGHLTVSFYSN

分子量

Approximately 31.9 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CS6 fimbrial subunit B/cssB Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CS6 fimbrial subunit B/cssB Protein, E.coli (His-SUMO)
目录号:
HY-P71473
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