CSF1R 蛋白, Human (HEK293)

CSF1R protein is ubiquitously found in mononuclear phagocytes, especially macrophages and monocytes, and is involved in a variety of biological processes. It induces the release of proinflammatory chemokines in response to IL34 and CSF1, contributing to innate immunity and inflammation. CSF1R Protein, Human (HEK293) is the recombinant human-derived CSF1R protein, expressed by HEK293 , with tag free. The total length of CSF1R Protein, Human (HEK293) is 493 a.a., with molecular weight of 80-120 kDa.

CSF1R 蛋白尤其存在于巨噬细胞和单核细胞等单核吞噬细胞中，参与多种生物过程。它响应 IL34 和 CSF1 促进促炎趋化因子的释放，有助于先天免疫和炎症过程。此外，CSF1R 对于调节破骨细胞增殖和分化、骨吸收、正常骨骼和牙齿发育至关重要。它也是男性和女性正常生育能力以及怀孕期间乳腺中乳管和腺泡结构发育所必需的。

1.Measured by its ability to inhibit the M-CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.01 μg/mL in the presence of 1 ng/mL of human M-CSF, corresponding to a specific activity is 1×10⁵ units/mg.
2.Immobilized Human M-CSF R, No Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC₅₀ of 7.1 ng/mL determined by ELISA.

• 
  Measured by its ability to inhibit the M-CSF-induced proliferation of M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 0.01 μg/mL in the presence of 1 ng/mL of human M-CSF, corresponding to a specific activity is 1×10⁵ units/mg.

Human

HEK293

Tag Free

P07333/NP_005202.2 (I20-E512)

1436  [NCBI]

IPVIEPSVPELVVKPGATVTLRCVGNGSVEWDGPPSPHWTLYSDGSSSILSTNNATFQNTGTYRCTEPGDPLGGSAAIHLYVKDPARPWNVLAQEVVVFEDQDALLPCLLTDPVLEAGVSLVRVRGRPLMRHTNYSFSPWHGFTIHRAKFIQSQDYQCSALMGGRKVMSISIRLKVQKVIPGPPALTLVPAELVRIRGEAAQIVCSASSVDVNFDVFLQHNNTKLAIPQQSDFHNNRYQKVLTLNLDQVDFQHAGNYSCVASNVQGKHSTSMFFRVVESAYLNLSSEQNLIQEVTVGEGLNLKVMVEAYPGLQGFNWTYLGPFSDHQPEPKLANATTKDTYRHTFTLSLPRLKPSEAGRYSFLARNPGGWRALTFELTLRYPPEVSVIWTFINGSGTLLCAASGYPQPNVTWLQCSGHTDRCDEAQVLQVWDDPYPEVLSQEPFHKVTVQSLLTVETLEHNQTYECRAHNSVGSGSWAFIPISAGAHTHPPDE

80-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.