1. 重组蛋白
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  3. CSF & Receptors
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  5. CSF3 蛋白, Rhesus macaque

CSF3 蛋白, Rhesus macaque

目录号: HY-P71877
COA 产品使用指南

CSF3 蛋白是 IL-6 超家族的成员,是影响造血的重要粒细胞/巨噬细胞集落刺激因子。它调节粒细胞和单核巨噬细胞的产生、分化和功能,在造血过程的复杂平衡中发挥关键作用。CSF3 在 IL-6 超家族中的重要性延伸到协调免疫系统组件以获得最佳功能。CSF3 蛋白, Rhesus macaque 是重组的 CSF3 蛋白,由 E. coli 表达,不带标签。CSF3 蛋白, Rhesus macaque 全长 177 个氨基酸,分子量约为 ~18.9 kDa。

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规格 价格 是否有货 数量
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2 μg ¥500 In-stock
10 μg ¥1400 In-stock
50 μg ¥3900 In-stock
100 μg ¥6650 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CSF3 Protein, a member of the IL-6 superfamily, is a vital granulocyte/macrophage colony-stimulating factor influencing hematopoiesis. It regulates the production, differentiation, and function of granulocytes and monocytes-macrophages, playing a key role in the intricate balance of hematopoietic processes. CSF3's significance within the IL-6 superfamily extends to orchestrating immune system components for optimal functionality. CSF3 Protein, Rhesus macaque is the recombinant Rhesus Macaque-derived CSF3 protein, expressed by E. coli , with tag free. The total length of CSF3 Protein, Rhesus macaque is 177 a.a., with molecular weight of ~18.9 kDa.

研究背景

CSF3蛋白属于IL-6超家族,是一种粒细胞/巨噬细胞集落刺激因子,在造血中发挥着至关重要的作用。这种细胞因子控制血液中两个相互连接的白细胞群(即粒细胞和单核细胞-巨噬细胞)的产生、分化和功能。具体来说,CSF3 诱导粒细胞的产生,有助于造血过程的复杂调节。作为 IL-6 超家族的关键成员,CSF3 在协调免疫系统内重要成分的平衡和功能方面具有重要意义。

生物活性

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NFS-60 cells is 1.5-50 pg/mL, corresponding to a specific activity of >2.0x107 IU/mg.

种属

Rhesus Macaque

表达系统

E. coli

标签

Tag Free

蛋白编号

F7H1Q6 (T31-S207)

基因 ID

/

蛋白结构
N-term
CSF3 (T31-S207)
Accession # F7H1Q6
C-term
中文名
重组猴CSF3蛋白
同用名
Granulocyte colony-stimulating factor; CSF3
氨基酸序列

TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLRHSLGIPWAPLSSCPSQALQLTGCLSQLHSSLFLYQGLLQALEGISPELSPTLDTLQLDIADFATTIWQQMEDLGMAPALQPTQGAMPAFTSAFQRRAGGVLVASHLQRFLELAYRVLRHLAQS

分子量

Approximately 18.9 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in sterile distilled water. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

CSF3 Protein, Rhesus macaque 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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