2. 重组蛋白
  3. Immune Checkpoint Proteins CD Antigens
  4. Inhibitory Checkpoint Molecules T Cell CD Proteins Macrophage CD Proteins Dendritic Cell CD Proteins
  5. CTLA-4 CD152/CTLA-4
  6. CTLA-4 蛋白, Canine (HEK293, His)

CTLA-4 蛋白, Canine (HEK293, His)

目录号: HY-P75320
COA 产品使用指南

CTLA-4 蛋白作为关键负调节因子抑制 T 细胞反应，对 CD80 和 CD86 的亲和力比 CD28 更强。这种亲和力使 CTLA-4 能够有效抑制 T 细胞活化并调节免疫反应，维持 T 细胞介导的免疫的微妙平衡。CTLA-4 蛋白, Canine (HEK293, His) 是重组的 CTLA-4 蛋白，由 HEK293 表达，带有 C-His 标签。CTLA-4 蛋白, Canine (HEK293, His) 全长 161 个氨基酸，分子量约为 19-25 kDa。

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3.  试用装只面向终端客户。

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5 μg ￥470 In-stock
10 μg ￥750 In-stock
50 μg ￥1950 In-stock
100 μg ￥3100 In-stock
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Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

CTLA-4 protein inhibits T-cell responses as a critical negative regulator, with a stronger affinity for CD80 and CD86 than CD28. This affinity allows CTLA-4 to effectively suppress T-cell activation and regulate immune responses, maintaining a delicate equilibrium in T-cell-mediated immunity. CTLA-4 Protein, Canine (HEK293, His) is the recombinant canine-derived CTLA-4 protein, expressed by HEK293 , with C-His labeled tag. The total length of CTLA-4 Protein, Canine (HEK293, His) is 161 a.a., with molecular weight of 19-25 kDa.
研究背景

CTLA-4 蛋白作为关键的抑制性受体发挥作用，作为 T 细胞反应的主要负调节因子发挥着关键作用。CTLA-4 的显着特征在于其对其天然 B7 家族配体 CD80 和 CD86 的亲和力明显强于其相应刺激辅助受体 CD28 的亲和力。这种增强的亲和力使 CTLA-4 能够有效平衡和抑制 T 细胞激活，从而有助于免疫反应的复杂调节。CTLA-4 及其配体之间的动态相互作用强调了其在微调免疫系统以及维持 T 细胞介导的免疫激活和抑制之间的微妙平衡方面的重要性。
生物活性

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 76.8 ng/mL, corresponding to a specific activity is 1.3×104 U/mg.
种属

Canine
表达系统

HEK293
标签

C-6*His
蛋白编号

Q9GKP2 (K36-D161)
基因 ID
蛋白结构
N-term
CTLA-4 (M1-D161)
Accession # Q9GKP2
His
C-term
同用名
Cytotoxic T-lymphocyte associated protein 4; CTLA4; CD152
氨基酸序列

KGMHVAQPAVVLASSRGVASFVCEYGSSGNAAEVRVTVLRQAGSQMTEVCAATYTVEDELAFLDDSTCTGTSSGNKVNLTIQGLRAMDTGLYICKVELMYPPPYYVGMGNGTQIYVIDPEPCPDSD

分子量

Approximately 19-25 kDa due to the glycosylation.
纯度

Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末.
组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.
复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
运输条件

Room temperature in continental US; may vary elsewhere.
文件资料

COA (148 KB)

产品使用指南 (1213 KB)

CTLA-4 Protein, Canine (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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