CTLA-4 蛋白, Human (HEK293, GST)

CTLA-4 Protein, Human (HEK293, GST) is negative regulator of T-cell immune function.

CTLA-4 是免疫球蛋白相关受体家族的成员，负责 T 细胞免疫调节的各个方面。该家族包括 CD28、CTLA-4 和 ICOS 以及其他蛋白，包括 PD-1、BTLA 和 TIGIT。CTLA-4 主要存在于 FoxP3⁺ Treg 细胞或激活的常规 T 细胞的细胞内囊泡中。CTLA-4 可以与 CD28 竞争配体结合，从而充当 CD28 介导的共刺激的拮抗剂^{[1][2][3]< /sup>。}

Human

HEK293

C-GST

P16410 (K36-D161)

1493  [NCBI]

KAMHVAQPAVVLASSRGIASFVCEYASPGKATEVRVTVLRQADSQVTEVCAATYMMGNELTFLDDSICTGTSSGNQVNLTIQGLRAMDTGLYICKVELMYPPPYYLGIGNGTQIYVIDPEPCPDSD

40-55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.

• [1]. Harper K, et, al. CTLA-4 and CD28 activated lymphocyte molecules are closely related in both mouse and human as to sequence, message expression, gene structure, and chromosomal location. J Immunol. 1991 Aug 1;147(3):1037-44.  [Content Brief]

  [2]. Buchbinder EI, et, al. CTLA-4 and PD-1 Pathways: Similarities, Differences, and Implications of Their Inhibition. Am J Clin Oncol. 2016 Feb;39(1):98-106.  [Content Brief]

  [3]. Rowshanravan B, et, al. CTLA-4: a moving target in immunotherapy. Blood. 2018 Jan 4;131(1):58-67.  [Content Brief]