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  3. Hydrolases (EC 3)
  4. CTRB1 蛋白, Human (HEK293, His)

CTRB1 蛋白, Human (HEK293, His)

目录号: HY-P70005
COA 产品使用指南

胰凝乳蛋白酶原 B (CTRB1) 是胰腺蛋白水解酶的前体。CTRB1 在胰腺的腺泡细胞中合成,并分泌到小肠中水解活化,产生功能性酶。CTRB1 激活丝氨酸型内肽酶活性,参与对凋亡信号、营养物质、细胞因子和肽类激素的反应。CTRB1 位于溶酶体中,是胰腺炎的一个生物标志物。CTRB1 蛋白, Human (HEK293, His) 是重组的 CTRB1 蛋白,由 HEK293 表达,带有 C-6*His 标签。CTRB1 蛋白, Human (HEK293, His) 全长 245 个氨基酸,分子量为 28-30 kDa。

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规格 价格 是否有货 数量
10 μg ¥1070 In-stock
50 μg ¥3000 In-stock
100 μg   询价  

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描述

Chymotrypsinogen B (CTRB1) is the precursor of the pancreatic proteolytic enzymes. CTRB1 is synthesized in the acinar cells of the pancreas and secreted into the small intestine where it undergoes proteolytic activation to generate the functional enzyme. CTRB1 enables serine-type endopeptidase activity, being involved in response to apoptotic signal, nutrient, cytokine and peptide hormone. CTRB1 is located in lysosome and is a biomarker of pancreatitis. CTRB1 Protein, Human (HEK293, His) is the recombinant human-derived CTRB1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CTRB1 Protein, Human (HEK293, His) is 245 a.a., with molecular weight of 28-30 kDa.

研究背景

胰凝乳蛋白酶原 B (CTRB1) 是丝氨酸蛋白酶家族的一员,是胰腺蛋白水解酶的主要前体。CTRB1 在胰腺的腺泡细胞中合成,并分泌到小肠中,在小肠中进行蛋白水解活化,产生功能性酶。CTRB1 激活丝氨酸型内肽酶活性,参与对凋亡信号、营养物质、细胞因子和肽类激素的反应。CTRB1 通过 Bid 切割活性诱导细胞凋亡,因为截断的 Bid 可导致线粒体快速释放细胞色素 C。CTRB1 位于溶酶体中,是胰腺炎的一个生物标志物。CTRB1 基因编码不同的异构体,这些异构体可能经历类似的加工以产生成熟的蛋白质[1][2][3][4][5]

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

P17538 (C19-N263)

基因 ID
蛋白结构
N-term
CTRB1 (C19-N263)
Accession # P17538
6*His
C-term
同用名
rHuChymotrypsinogen B/CTRB1, His; Chymotrypsinogen B; CTRB1; CTRB
氨基酸序列

CGVPAIHPVLSGLSRIVNGEDAVPGSWPWQVSLQDKTGFHFCGGSLISEDWVVTAAHCGVRTSDVVVAGEFDQGSDEENIQVLKIAKVFKNPKFSILTVNNDITLLKLATPARFSQTVSAVCLPSADDDFPAGTLCATTGWGKTKYNANKTPDKLQQAALPLLSNAECKKSWGRRITDVMICAGASGVSSCMGDSGGPLVCQKDGAWTLVGIVSWGSDTCSTSSPGVYARVTKLIPWVQKILAAN

分子量

28-30 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

CTRB1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CTRB1 Protein, Human (HEK293, His)
目录号:
HY-P70005
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