1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins
  3. Chemokine & Receptors G-Protein-Coupled Receptors (GPCRs)
  4. CXC Chemokine Receptor Chemokine Receptor
  5. CXCR2
  6. CXCR2 蛋白, Mouse (Cell-Free, His)

CXCR2 蛋白, Mouse (Cell-Free, His)

目录号: HY-P702257
产品使用指南

CXCR2 蛋白作为白细胞介素 8 的受体,通过 G 蛋白介导的磷脂酰肌醇-钙第二信使系统诱导中性粒细胞活化。除了 IL-8 之外,CXCR2 还表现出与 CXCL3、GRO/MGSA 和 NAP-2 等配体的高亲和力结合。CXCR2 蛋白, Mouse (Cell-Free, His) 是重组的 CXCR2 蛋白,由 E. coli Cell-free 表达,带有 N-10*His 标签。CXCR2 蛋白, Mouse (Cell-Free, His) 全长 359 个氨基酸,分子量为 43.2 kDa。

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     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

CXCR2 protein acts as a receptor for interleukin-8 and induces neutrophil activation through the G protein-mediated phosphatidylinositol-calcium second messenger system. In addition to IL-8, CXCR2 also exhibits high-affinity binding to ligands such as CXCL3, GRO/MGSA, and NAP-2. CXCR2 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived CXCR2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of CXCR2 Protein, Mouse (Cell-Free, His) is 359 a.a., with molecular weight of 43.2 kDa.

研究背景

CXCR2 蛋白是白细胞介素 8(一种有效的中性粒细胞趋化因子)的受体,其与受体的结合导致中性粒细胞的激活。这种反应是通过 G 蛋白介导的,启动磷脂酰肌醇-钙第二信使系统。CXCR2 不仅与 IL-8 具有高亲和力结合,还与其他配体(包括 CXCL3、GRO/MGSA 和 NAP-2)具有高亲和力结合。该蛋白直接与 IL8 相互作用并与 GNAI2 相关,突出了其在涉及中性粒细胞活性和趋化性调节的复杂信号通路中的作用。这些相互作用强调了 CXCR2 在介导免疫反应中的重要性,特别是与各种生理和病理环境中中性粒细胞的招募和激活相关的免疫反应。

种属

Mouse

表达系统

E. coli Cell-free

标签

N-10*His

蛋白编号

P35343 (M1-L359)

基因 ID

12765

蛋白结构
N-term
10*His
CXCR2 (M1-L359)
Accession # P35343
C-term
同用名
C-X-C chemokine receptor type 2; GRO/MGSA receptor; High affinity interleukin-8 receptor B; IL-8R B; CD182
氨基酸序列

MGEFKVDKFNIEDFFSGDLDIFNYSSGMPSILPDAVPCHSENLEINSYAVVVIYVLVTLLSLVGNSLVMLVILYNRSTCSVTDVYLLNLAIADLFFALTLPVWAASKVNGWTFGSTLCKIFSYVKEVTFYSSVLLLACISMDRYLAIVHATSTLIQKRHLVKFVCIAMWLLSVILALPILILRNPVKVNLSTLVCYEDVGNNTSRLRVVLRILPQTFGFLVPLLIMLFCYGFTLRTLFKAHMGQKHRAMRVIFAVVLVFLLCWLPYNLVLFTDTLMRTKLIKETCERRDDIDKALNATEILGFLHSCLNPIIYAFIGQKFRHGLLKIMATYGLVSKEFLAKEGRPSFVSSSSANTSTTL

分子量

43.2 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CXCR2 Protein, Mouse (Cell-Free, His)
目录号:
HY-P702257
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