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  4. CXCR5/CD185 Protein-VLP, Human (HEK293)

CXCR5/CD185 Protein-VLP, Human (HEK293)

目录号: HY-P704224
产品使用指南 技术支持

CXCR5/CD185 Protein-VLP, Human (HEK293) 是重组人源的 CXCR5/CD185 蛋白，由 HEK293 表达，带有 C-His 标签。VLPs在HEK293细胞培养中形成，将膜蛋白集中在细胞表面，产生适合免疫和抗体筛选的高浓度蛋白。VLPs免疫原性强，需优化免疫策略，包括抗原剂量、接种方案和佐剂选择。本产品不推荐应用于受配体互作实验和SPR/BLI分析, 因为VLP包膜上还有宿主其他无关膜蛋白, 受配体互作会有很强的背景干扰. SPR/BLI分析则对芯片和实验方案有较高要求，成功率较低. 如果需要VLP对照，推荐HY-P705433。

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SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货
20 μg ￥7350 询问价格 & 货期
100 μg ￥14700 询问价格 & 货期

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描述

CXCR5/CD185 Protein-VLP, Human (HEK293) is the recombinant human-derived CXCR5/CD185 protein, expressed by HEK293, with C-His tag.Formed in HEK293 cultures, these VLPs concentrate membrane proteins on the cell surface, producing high-concentration proteins ideal for immunization and antibody screening. Given the high immunogenicity of VLPs, the immunization protocol such as antigen dose, schedule, and adjuvant, requires optimization. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P705433. Tags can only be detected under denaturing conditions.
研究背景

CXCR5 是一种细胞因子受体，能够与 B 淋巴细胞趋化因子 (BLC) 结合。它参与 B 细胞向脾脏和 Peyer 斑的 B 细胞滤泡迁移，但不参与肠系膜或外周淋巴结的 B 细胞滤泡迁移。可能在 Burkitt 淋巴瘤 (BL) 的淋巴瘤发生和/或 B 细胞分化中具有调节功能。
种属

Human
表达系统

HEK293
标签

C-His;Tag Free
蛋白编号

P32302-1 (N2-F372)
基因 ID

643
蛋白结构
N-term
CXCR5 (N2-F372)
Accession # P32302-1
C-term
蛋白长度

Full Length of Isoform-1
同用名
BLR1; L CD185; L MDR15; LC-X-C chemokine receptor type 5; LBurkitt lymphoma receptor 1; LMonocyte-derived receptor 15 (MDR-15)
糖基化修饰
Yes
构象/形态
Monomer
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

CXCR5/CD185 Protein-VLP, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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