CYB5R1 蛋白, Human (His)

CYB5R1 is an important member of the NADH-cytochrome b5 reductase family and plays a crucial role in fatty acid desaturation, cholesterol biosynthesis, drug metabolism, and erythrocyte methemoglobin reduction. Utilizing NADH, CYB5R1 promotes the reduction of cytochrome b5, contributing to enzymatic processes in lipid metabolism, sterol synthesis, and drug biotransformation. CYB5R1 Protein, Human (His) is the recombinant human-derived CYB5R1 protein, expressed by E. coli , with N-His labeled tag. The total length of CYB5R1 Protein, Human (His) is 277 a.a., with molecular weight of ~33.5 kDa.

CYB5R1 是 NADH-细胞色素 b5 还原酶家族的成员，在多种生物过程中发挥着至关重要的作用，例如脂肪酸去饱和和延伸、胆固醇生物合成、药物代谢和红细胞中高铁血红蛋白还原。作为这些代谢途径的重要组成部分，CYB5R1 利用 NADH 作为辅助因子来促进细胞色素 b5 的还原，有助于参与脂质代谢、甾醇合成和药物生物转化的酶促过程。在红细胞中，CYB5R1 参与高铁血红蛋白减少，强调了其在维持血红蛋白功能和预防氧化应激方面的重要性。CYB5R1 的多功能性质使其成为对脂质稳态、血红蛋白功能和药物代谢至关重要的细胞过程中的关键参与者。

Measured by its ability to combine with the NADPH produces the H₂O₂. The specific activity is 7661.210 pmoL/min/μg, as measured under the described conditions.

Human

E. coli

N-His

Q9UHQ9 (L29-Y305)

51706  [NCBI]

LVRRSRRPQVTLLDPNEKYLLRLLDKTTVSHNTKRFRFALPTAHHTLGLPVGKHIYLSTRIDGSLVIRPYTPVTSDEDQGYVDLVIKVYLKGVHPKFPEGGKMSQYLDSLKVGDVVEFRGPSGLLTYTGKGHFNIQPNKKSPPEPRVAKKLGMIAGGTGITPMLQLIRAILKVPEDPTQCFLLFANQTEKDIILREDLEELQARYPNRFKLWFTLDHPPKDWAYSKGFVTADMIREHLPAPGDDVLVLLCGPPPMVQLACHPNLDKLGYSQKMRFTY

Approximately 32 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.