1. 重组蛋白
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. Cyclophilin A 蛋白, Mouse (His)

Cyclophilin A 蛋白, Mouse (His)

目录号: HY-P74212
COA 产品使用指南

亲环蛋白 A (PPIA) 蛋白具有整合素结合和肽基脯氨酰顺反异构酶活性,影响血小板聚集和神经元分化。它存在于细胞外空间和髓鞘中,在多种组织中普遍表达,包括肝脏和中枢神经系统。PPIA 与胆管癌和 HIV 疾病相关,表明其在基本细胞过程中的作用。Cyclophilin A 蛋白, Mouse (His) 是重组的 亲环蛋白 A 蛋白,由 E. coli 表达,带有 C-His 标签。Cyclophilin A 蛋白, Mouse (His) 全长 164 个氨基酸,分子量约为 ~18.94 kDa。

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规格 价格 是否有货 数量
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5 μg ¥450 In-stock
10 μg ¥780 In-stock
50 μg ¥2280 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Cyclophilin A (PPIA) protein, with integrin binding and peptidyl-prolyl cis-trans isomerase activity, influences platelet aggregation and neuron differentiation. It is found in the extracellular space and myelin sheath, showing ubiquitous expression in various tissues, including the liver and central nervous system. PPIA is associated with cholangiocarcinoma and HIV disease, suggesting its role in fundamental cellular processes. Cyclophilin A Protein, Mouse (His) is the recombinant mouse-derived Cyclophilin A protein, expressed by E. coli , with C-His labeled tag. The total length of Cyclophilin A Protein, Mouse (His) is 164 a.a., with molecular weight of ~18.94 kDa.

研究背景

亲环蛋白 A (PPIA) 蛋白具有整联蛋白结合活性和肽基-脯氨酰顺反异构酶活性。这种多功能蛋白质参与血小板聚集,并在神经元分化相关过程的上游发挥作用。亲环蛋白 A 位于细胞外空间和髓鞘中,有助于其功能多样性。编码这种蛋白质的基因在各种组织中普遍表达,包括肝脏、中枢神经系统和早期发育阶段的许多其他结构。人类直向同源物 PPIA 的影响包括其与胆管癌和人类免疫缺陷病毒感染性疾病的关联。这种广泛的表达模式表明亲环蛋白 A 参与多个组织的基本细胞过程。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Mouse

表达系统

E. coli

标签

C-His

蛋白编号

NP_032933.1 (M1-L164)

基因 ID

268373  [NCBI]

蛋白结构
N-term
Cyclophilin A (M1-L164)
Accession # NP_032933.1
His
C-term
同用名
Peptidyl-prolyl cis-trans isomerase A; PPIase A; SP18; PPIA; CYPA
氨基酸序列

MVNPTVFFDITADDEPLGRVSFELFADKVPKTAENFRALSTGEKGFGYKGSSFHRIIPGFMCQGGDFTRHNGTGGRSIYGEKFEDENFILKHTGPGILSMANAGPNTNGSQFFICTAKTEWLDGKHVVFGKVKEGMNIVEAMERFGSRNGKTSKKITISDCGQL

分子量

Approximately 18.94 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4, 10% glycerol or 20 mM PB, 150 mM NaCl, 10% Glycerol, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Cyclophilin A Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Cyclophilin A Protein, Mouse (His)
目录号:
HY-P74212
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