1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. CYP102A1 蛋白, Priestia megaterium (P. pastoris, His)

CYP102A1 蛋白, Priestia megaterium (P. pastoris, His)

目录号: HY-P700629
产品使用指南

CYP102A1 蛋白充当脂肪酸单加氧酶,催化脂肪酸在各个位置的羟基化。该蛋白还表现出 NADPH 依赖性还原酶活性,促进其结构域内的电子转移。CYP102A1 蛋白, Priestia megaterium (P. pastoris, His) 是重组的 CYP102A1 蛋白,由 P. pastoris 表达,带有 C-6*His 标签。CYP102A1 蛋白, Priestia megaterium (P. pastoris, His) 全长 471 个氨基酸,分子量为 55.2 kDa。

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描述

The CYP102A1 protein acts as a fatty acid monooxygenase, catalyzing the hydroxylation of fatty acids at various positions. The protein also exhibits NADPH-dependent reductase activity, promoting electron transfer within its domain. CYP102A1 Protein, Priestia megaterium (P. pastoris, His) is the recombinant CYP102A1 protein, expressed by P. pastoris , with C-6*His labeled tag. The total length of CYP102A1 Protein, Priestia megaterium (P. pastoris, His) is 471 a.a., with molecular weight of 55.2 kDa.

研究背景

CYP102A1 被称为脂肪酸单加氧酶,具有多种酶活性,有助于其在多种代谢过程中发挥作用。该蛋白质催化不同 omega 位点脂肪酸的羟基化,包括 omega-1、omega-2 和 omega-3,特别是中链和长链脂肪酸,如月桂酸、肉豆蔻酸和棕榈酸。此外,CYP102A1可以将一些初级脂肪酸代谢物代谢成二级和三级产物。值得注意的是,它对短链脂肪酸表现出边际活性。其羟基化高度支化脂肪酸的能力对于调节膜流动性至关重要。除了单加氧酶功能外,CYP102A1 在 C 端结构域中还表现出 NADPH 依赖性还原酶活性,促进电子转移到细胞色素 P450 N 端结构域的血红素铁。其功能的一个有趣的方面涉及竞争细菌产生的群体感应信号的失活。CYP102A1 有效氧化酰基高丝氨酸内酯 (AHL)(群体感应信号通路中的关键分子)及其内酯分解产物酰基高丝氨酸 (AH),从而有助于细菌通讯的调节。

种属

Others

表达系统

P. pastoris

标签

C-6*His

蛋白编号

P14779 (T2-R472)

基因 ID

/

蛋白结构
N-term
CYP102A1 (T2-R472)
Accession # P14779
6*His
C-term
同用名
Bifunctional cytochrome P450; NADPH--P450 reductase; Cytochrome P450BM-3; Fatty acid monooxygenase; Flavocytochrome P450 BM3
氨基酸序列

TIKEMPQPKTFGELKNLPLLNTDKPVQALMKIADELGEIFKFEAPGRVTRYLSSQRLIKEACDESRFDKNLSQALKFVRDFAGDGLFTSWTHEKNWKKAHNILLPSFSQQAMKGYHAMMVDIAVQLVQKWERLNADEHIEVPEDMTRLTLDTIGLCGFNYRFNSFYRDQPHPFITSMVRALDEAMNKLQRANPDDPAYDENKRQFQEDIKVMNDLVDKIIADRKASGEQSDDLLTHMLNGKDPETGEPLDDENIRYQIITFLIAGHETTSGLLSFALYFLVKNPHVLQKAAEEAARVLVDPVPSYKQVKQLKYVGMVLNEALRLWPTAPAFSLYAKEDTVLGGEYPLEKGDELMVLIPQLHRDKTIWGDDVEEFRPERFENPSAIPQHAFKPFGNGQRACIGQQFALHEATLVLGMMLKHFDFEDHTNYELDIKETLTLKPEGFVVKAKSKKIPLGGIPSPSTEQSAKKVR

分子量

55.2 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CYP102A1 Protein, Priestia megaterium (P. pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CYP102A1 Protein, Priestia megaterium (P. pastoris, His)
目录号:
HY-P700629
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