CYP21A2 蛋白, Human (P.pastoris, His)

CYP21A2 Protein, a cytochrome P450 monooxygenase, crucially hydroxylates progesterone and 17alpha-hydroxyprogesterone at C-21, yielding vital intermediates for mineralocorticoid and glucocorticoid biosynthesis in adrenal steroidogenesis. This enzymatic process involves molecular oxygen, with NADPH providing essential electrons via cytochrome P450 reductase (CPR). CYP21A2 plays a pivotal role in regulating adrenal cortex hormone production. CYP21A2 Protein, Human (P.pastoris, His) is the recombinant human-derived CYP21A2 protein, expressed by P. pastoris, with N-6*His labeled tag. The total length of CYP21A2 Protein, Human (P.pastoris, His) is 494 a.a., with molecular weight of ~57.9 kDa.

CYP21A2 蛋白是一种细胞色素 P450 单加氧酶，通过催化孕酮和 17α-羟基孕酮的 C-21 羟基化，在肾上腺类固醇生成中发挥关键作用。这种酶活性导致关键中间代谢物的形成，即 11-脱氧皮质酮和 11-脱氧皮质醇，它们是盐皮质激素和糖皮质激素生物合成途径中不可或缺的组成部分。从机制上讲，CYP21A2 利用分子氧，将一个氧原子插入底物中，并将第二个氧原子还原成水分子。此过程所需的电子由 NADPH 通过细胞色素 P450 还原酶（CPR；NADPH-高铁血蛋白还原酶）提供。CYP21A2 协调的这些生化转化对于关键肾上腺类固醇的合成至关重要，对肾上腺皮质中盐皮质激素和糖皮质激素产生的调节做出了重大贡献。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

P. pastoris

N-6*His

P08686 (1M-494Q)

1589  [NCBI]

MLLLGLLLLPLLAGARLLWNWWKLRSLHLPPLAPGFLHLLQPDLPIYLLGLTQKFGPIYRLHLGLQDVVVLNSKRTIEEAMVKKWADFAGRPEPLTYKLVSKNYPDLSLGDYSLLWKAHKKLTRSALLLGIRDSMEPVVEQLTQEFCERMRAQPGTPVAIEEEFSLLTCSIICYLTFGDKIKDDNLMPAYYKCIQEVLKTWSHWSIQIVDVIPFLRFFPNPGLRRLKQAIEKRDHIVEMQLRQHKESLVAGQWRDMMDYMLQGVAQPSMEEGSGQLLEGHVHMAAVDLLIGGTETTANTLSWAVVFLLHHPEIQQRLQEELDHELGPGASSSRVPYKDRARLPLLNATIAEVLRLRPVVPLALPHRTTRPSSISGYDIPEGTVIIPNLQGAHLDETVWERPHEFWPDRFLEPGKNSRALAFGCGARVCLGEPLARLELFVVLTRLLQAFTLLPSGDALPSLQPLPHCSVILKMQPFQVRLQPRGMGAHSPGQNQ

Approximately 57.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.