DAP10 蛋白, Human (HEK293, mFc)

DAP10 is a transmembrane adapter protein that forms the activating receptor KLRK1-HCST, which is critical for cytotoxicity in target cells expressing stress-inducing ligands. It is activated by ULBP, triggering calcium mobilization and activating PI3K, MAP2K/ERK and JAK2/STAT5 signaling pathways. DAP10 Protein, Human (HEK293, mFc) is the recombinant human-derived DAP10 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of DAP10 Protein, Human (HEK293, mFc) is 30 a.a., with molecular weight of ~33-45 kDa.

DAP10 是一种跨膜衔接蛋白，在淋巴和骨髓细胞中形成激活受体 KLRK1-HCST，在触发针对表达应激诱导配体（例如 MHC I 类链相关 MICA 和 MICB）的靶细胞的细胞毒性方面发挥着关键作用，如以及 UL16 结合蛋白 (ULBP)。该受体由 ULBP 与 KLRK1-HCST 相互作用激活，启动钙动员并激活 PI3 激酶 PIK3R1、MAP2K/ERK 和 JAK2/STAT5 信号通路。PIK3R1 和 GRB2 的存在对于 KLRK1-HCST 介导的完全激活至关重要，从而最终杀死靶细胞。在 NK 细胞中，KLRK1-HCST 信号传导诱导细胞毒性并增强细胞因子的产生，而在 T 细胞中，它主要为 TCR 诱导的信号提供共刺激。KLRK1-HCST 受体在肿瘤免疫监视中发挥着至关重要的作用，其缺失会导致黑色素瘤细胞逃避 NK 细胞介导的免疫监视。DAP10 与 CLEC5A 相互作用，形成 CLEC5A/TYROBP/HCST 三分子复合物，并通过二硫键形成同二聚体。该异六聚体包含四个 HCST/DAP10 亚基和两个 KLRK1 亚基，它与 PIK3R1、GRB2、KLRK1 和 CD300H 相互作用。

Human

HEK293

C-mFc

Q9UBK5 (Q19-P48)

10870  [NCBI]

QTTPGERSSLPAFYPGTSGSCSGCGSLSLP

Approximately 33-45 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.