1. 重组蛋白
  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. DARS 蛋白, Human (His)

DARS 蛋白, Human (His)

目录号: HY-P75309
COA 产品使用指南

DARS 蛋白是一个核心角色,催化 ATP 和 AMP 之间末端磷酸基团的可逆转移,维持细胞能量稳态。它还表现出核苷二磷酸激酶活性,产生各种核苷三磷酸。DARS 以较低的速率参与二磷酸硫胺素和 ADP 合成三磷酸硫胺素,显示出其在核苷酸代谢中的多方面作用。DARS 蛋白, Human (His) 是重组的 DARS 蛋白,由 E. coli 表达,带有 N-His 标签。DARS 蛋白, Human (His) 全长 501 个氨基酸,分子量约为 ~47 kDa。

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描述

DARS Protein, a central player, catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP, maintaining cellular energy homeostasis. It also exhibits nucleoside diphosphate kinase activity, producing various nucleoside triphosphates. At a low rate, DARS participates in thiamine triphosphate synthesis from thiamine diphosphate and ADP, showcasing its multifaceted role in nucleotide metabolism. DARS Protein, Human (His) is the recombinant human-derived DARS protein, expressed by E. coli , with N-His labeled tag. The total length of DARS Protein, Human (His) is 501 a.a., with molecular weight of ~47 kDa.

研究背景

腺苷酸激酶 1 (AK1) 蛋白通过催化 ATP 和 AMP 之间末端磷酸基团的可逆转移,在细胞能量稳态中发挥关键作用。此外,它还具有核苷二磷酸激酶活性,能够使用 ATP 或 GTP 作为磷酸盐供体,从相应的二磷酸底物中生成各种核苷三磷酸,包括 ATP、CTP、GTP、UTP、dATP、dCTP、dGTP 和 dTTP 。此外,AK1虽然以非常低的速率参与从二磷酸硫胺素(ThDP)和ADP合成三磷酸硫胺素(ThTP),展示了其在核苷酸代谢中的多方面酶活性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

E. coli

标签

N-His

蛋白编号

P14868 (M1-P501)

基因 ID
蛋白结构
N-term
His
DARS (M1-P501)
Accession # P14868
C-term
同用名
Aspartate--tRNA ligase, cytoplasmic; AspRS; DARS1; PIG40
分子量

Approximately 47 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

溶液

组分

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Glycerol, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

DARS Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
DARS Protein, Human (His)
目录号:
HY-P75309
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