1. 重组蛋白
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. DCT 蛋白, Mouse (His-SUMO)

DCT 蛋白, Mouse (His-SUMO)

目录号: HY-P72169
产品使用指南

DCT(多巴色素互变异构酶)在黑色素生物合成中至关重要,可催化 L-多巴色素转化为 5,6-二羟基吲哚-2-羧酸 (DHICA),这是黑色素合成的关键步骤。DCT 的酶活性是控制黑色素生成的复杂过程中不可或缺的一部分,强调其在皮肤色素沉着和黑色素相关功能调节中的作用。DCT 蛋白, Mouse (His-SUMO) 是重组的 DCT 蛋白,由 E. coli 表达,带有 N-10*His, N-SUMO 标签。DCT 蛋白, Mouse (His-SUMO) 全长 449 个氨基酸,分子量约为 ~69.7 kDa。

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描述

DCT (Dopachrome tautomerase) is pivotal in melanin biosynthesis, catalyzing the conversion of L-dopachrome to 5,6-dihydroxyindole-2-carboxylic acid (DHICA), a critical step in melanin synthesis. DCT's enzymatic activity is integral to the complex processes governing melanin production, emphasizing its role in skin pigmentation and the regulation of melanin-related functions. DCT Protein, Mouse (His-SUMO) is the recombinant mouse-derived DCT protein, expressed by E. coli , with N-10*His, N-SUMO labeled tag. The total length of DCT Protein, Mouse (His-SUMO) is 449 a.a., with molecular weight of ~69.7 kDa.

研究背景

DCT(多巴色素互变异构酶)是黑色素生物合成不可或缺的一部分,黑色素生物合成是色素沉着的关键过程。作为一种酶,DCT 在 L-多巴色素转化为 5,6-二羟基吲哚-2-羧酸 (DHICA) 的过程中发挥着关键作用,这是黑色素合成途径的关键步骤。DCT 的催化活性有助于黑色素产生的复杂生化过程,突显其在决定皮肤色素沉着和调节黑色素相关生物功能方面的重要性。

种属

Mouse

表达系统

E. coli

标签

N-10*His;N-SUMO

蛋白编号

P29812 (Q24-S472)

基因 ID

13190  [NCBI]

蛋白结构
N-term
10*His-SUMO
DCT (Q24-S472)
Accession # P29812
C-term
同用名
Dct; Tyrp-2; Tyrp2; L-dopachrome tautomerase; DCT; DT; EC 5.3.3.12; DOPAchrome conversion factor; DOPAchrome isomerase; DOPAchrome oxidoreductase; L-dopachrome Delta-isomerase; SLATY locus protein; Tyrosinase-related protein 2; TRP-2; TRP2
氨基酸序列

QFPRVCMTLDGVLNKECCPPLGPEATNICGFLEGRGQCAEVQTDTRPWSGPYILRNQDDREQWPRKFFNRTCKCTGNFAGYNCGGCKFGWTGPDCNRKKPAILRRNIHSLTAQEREQFLGALDLAKKSIHPDYVITTQHWLGLLGPNGTQPQIANCSVYDFFVWLHYYSVRDTLLGPGRPYKAIDFSHQGPAFVTWHRYHLLWLERELQRLTGNESFALPYWNFATGKNECDVCTDELLGAARQDDPTLISRNSRFSTWEIVCDSLDDYNRRVTLCNGTYEGLLRRNKVGRNNEKLPTLKNVQDCLSLQKFDSPPFFQNSTFSFRNALEGFDKADGTLDSQVMNLHNLAHSFLNGTNALPHSAANDPVFVVLHSFTDAIFDEWLKRNNPSTDAWPQELAPIGHNRMYNMVPFFPPVTNEELFLTAEQLGYNYAVDLSEEEAPVWSTTLS

分子量

Approximately 69.7 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

DCT Protein, Mouse (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
DCT Protein, Mouse (His-SUMO)
目录号:
HY-P72169
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