DDX19A 蛋白, Human (His-SUMO)

The DDX19A protein is an ATP-dependent RNA helicase that plays a crucial role in unwinding single- and double-stranded DNA in the 3'-5' direction. It is actively involved in DNA replication and repair by recruiting DNA2 to aid in 5' end resection during double-strand break repair. DDX19A Protein, Human (His-SUMO) is the recombinant human-derived DDX19A protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of DDX19A Protein, Human (His-SUMO) is 478 a.a., with molecular weight of ~70.0 kDa.

BLM 是一种 ATP 依赖性 DNA 解旋酶，具有在 3'-5' 方向解旋单链和双链 DNA 的能力。它积极参与 DNA 复制和修复过程，在双链断裂修复过程中通过解旋 DNA 和招募 DNA2 在 DNA 5' 端切除中发挥至关重要的作用，从而促进 5'-ssDNA 的裂解。此外，BLM 负向调节姐妹染色单体交换，并参与刺激 DNA 4 路连接分支迁移以及 DNA 霍利迪连接体溶解。这种多面蛋白可与单链 DNA、分叉双链 DNA 和 DNA 霍利迪连接体结合。值得注意的是，BLM 被 KHDC3-OOEP 支架招募到 DNA 复制叉，并通过 TRIM25 泛素化保留下来，从而促进停滞的复制叉重新启动。

Human

E. coli

N-6*His;N-SUMO

Q9NUU7 (1M-478N)

55308  [NCBI]

MATDSWALAVDEQEAAVKSMTNLQIKEEKVKADTNGIIKTSTTAEKTDEEEKEDRAAQSLLNKLIRSNLVDNTNQVEVLQRDPNSPLYSVKSFEELRLKPQLLQGVYAMGFNRPSKIQENALPMMLAEPPQNLIAQSQSGTGKTAAFVLAMLSRVEPSDRYPQCLCLSPTYELALQTGKVIEQMGKFYPELKLAYAVRGNKLERGQKISEQIVIGTPGTVLDWCSKLKFIDPKKIKVFVLDEADVMIATQGHQDQSIRIQRMLPRNCQMLLFSATFEDSVWKFAQKVVPDPNVIKLKREEETLDTIKQYYVLCSSRDEKFQALCNLYGAITIAQAMIFCHTRKTASWLAAELSKEGHQVALLSGEMMVEQRAAVIERFREGKEKVLVTTNVCARGIDVEQVSVVINFDLPVDKDGNPDNETYLHRIGRTGRFGKRGLAVNMVDSKHSMNILNRIQEHFNKKIERLDTDDLDEIEKIAN

Approximately 70.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.