Delta-like 蛋白 1/DLL1 蛋白, Human (HEK293, His)

DLL1 protein is a transmembrane ligand for NOTCH1, NOTCH2 and NOTCH3 receptors and participates in cis- and trans-interactions. Trans interactions trigger mechanical forces involving clathrin-mediated endocytosis, ligand ubiquitination, EPN1 interactions, and actin polymerization. Delta-like protein 1/DLL1 Protein, Human (HEK293, His) is the recombinant human-derived Delta-like protein 1/DLL1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Delta-like protein 1/DLL1 Protein, Human (HEK293, His) is 523 a.a., with molecular weight of ~65.0 kDa.

Delta 样蛋白 1 (DLL1) 作为 NOTCH1、NOTCH2 和 NOTCH3 受体的跨膜配体蛋白，与 Notch 受体的胞外结构域 (ECD) 进行顺式和反式相互作用。相互作用后，配体细胞通过网格蛋白介导的内吞作用施加机械力，包括配体泛素化、EPN1 相互作用和肌动蛋白聚合。这些事件促进Notch受体胞外域(NECD)转内吞作用，触发Notch信号传导，包括Notch受体(NICD)胞内域的裂解、过度磷酸化和核积累。DLL1 对于胚胎发育、维持各种组织和免疫系统中的成体干细胞至关重要。它参与细胞间通讯，调节细胞谱系、模式和形态发生，影响分化和增殖。在大脑发育中，DLL1 在神经元分化和新皮质发育中发挥作用，而在小脑发育中，它调节伯格曼神经胶质单层的形成。它还通过 Notch 信号通路促进免疫系统发育、肌肉发育、胰腺细胞发育、动脉特性维持、血管生成、杯状细胞分化、内耳发育和肾单位发育。DLL1 作为同二聚体发挥作用，并与各种蛋白质相互作用，协调多种细胞过程。

Human

HEK293

C-6*His

O00548 (Q18-G540)

28514  [NCBI]

QVWSSGVFELKLQEFVNKKGLLGNRNCCRGGAGPPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGGGADSAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLATQRHLTVGEEWSQDLHSSGRTDLKYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKVCNPGWKGPYCTEPICLPGCDEQHGFCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCKNGATCTNTGQGSYTCSCRPGYTGATCELGIDECDPSPCKNGGSCTDLENSYSCTCPPGFYGKICELSAMTCADGPCFNGGRCSDSPDGGYSCRCPVGYSGFNCEKKIDYCSSSPCSNGAKCVDLGDAYLCRCQAGFSGRHCDDNVDDCASSPCANGGTCRDGVNDFSCTCPPGYTGRNCSAPVSRCEHAPCHNGATCHERGHRYVCECARGYGGPNCQFLLPELPPGPAVVDLTEKLEGQG

Approximately 65.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Hepes, 150 mM NaCl, 1 mM EDTA, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.