Delta-like 蛋白 4/DLL4 蛋白, Human (HEK293, Fc)

The DLL4 protein, a homolog of the Drosophila delta gene family, encodes Notch ligands with a DSL domain, EGF repeats, and a transmembrane domain. DLL4 is widely expressed in tissues, including placenta (RPKM 11.5), fat (RPKM 11.2), and 22 others. Delta-like protein 4/DLL4 Protein, Human (HEK293, Fc) is the recombinant human-derived Delta-like protein 4/DLL4 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Delta-like protein 4/DLL4 Protein, Human (HEK293, Fc) is 498 a.a., with molecular weight of 100-110 kDa.

Delta 样蛋白 4 (DLL4) 是果蝇 delta 基因家族的同源物，编码具有 DSL 结构域、EGF 重复序列和跨膜结构域的 Notch 配体。DLL4 在多种组织中广泛表达，包括胎盘 (RPKM 11.5)、脂肪 (RPKM 11.2) 和 22 个其他组织。

1. Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells and the ED₅₀ is typically 2.2 µg/mL in the presence of 500 ng/mL recombinant human BMP2.
2. Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is approximately 0.05348 μg/ml in the presence of 100 ng/mL Mouse BMP-2, corresponding to a specific activity is 1.870×10^4 units/mg.

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  Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is approximately 0.05348μg/mL in the presence of 100 ng/mL Mouse BMP-2, corresponding to a specific activity is 1.870×10⁴ units/mg.

Human

HEK293

C-hFc

NP_061947.1 (S27-P524)

54567  [NCBI]

SGVFQLQLQEFINERGVLASGRPCEPGCRTFFRVCLKHFQAVVSPGPCTFGTVSTPVLGTNSFAVRDDSSGGGRNPLQLPFNFTWPGTFSLIIEAWHAPGDDLRPEALPPDALISKIAIQGSLAVGQNWLLDEQTSTLTRLRYSYRVICSDNYYGDNCSRLCKKRNDHFGHYVCQPDGNLSCLPGWTGEYCQQPICLSGCHEQNGYCSKPAECLCRPGWQGRLCNECIPHNGCRHGTCSTPWQCTCDEGWGGLFCDQDLNYCTHHSPCKNGATCSNSGQRSYTCTCRPGYTGVDCELELSECDSNPCRNGGSCKDQEDGYHCLCPPGYYGLHCEHSTLSCADSPCFNGGSCRERNQGANYACECPPNFTGSNCEKKVDRCTSNPCANGGQCLNRGPSRMCRCRPGFTGTYCELHVSDCARNPCAHGGTCHDLENGLMCTCPAGFSGRRCEVRTSIDACASSPCFNRATCYTDLSTDTFVCNCPYGFVGSRCEFPVGLP

100-110 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 (Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. ) or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.