2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. DHCR7 蛋白, Bovine (Cell-Free, His)

DHCR7 蛋白, Bovine (Cell-Free, His)

目录号: HY-P702263
产品使用指南

DHCR7 蛋白，又名 7-脱氢胆固醇还原酶，通过还原中间体——cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) 和 cholesta- 中的 C7-C8 双键，至关重要地参与胆固醇生物合成途径。5,7,24-三烯-3β-醇。DHCR7 蛋白, Bovine (Cell-Free, His) 是重组的 DHCR7 蛋白，由 E. coli Cell-free 表达，带有 N-10*His 标签。DHCR7 蛋白, Bovine (Cell-Free, His) 全长 475 个氨基酸，分子量为 55.8 kDa。

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规格 是否有货
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100 μg   询价  

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描述

DHCR7 Protein, alias 7-dehydrocholesterol reductase, crucially participates in the cholesterol biosynthetic pathway by reducing the C7-C8 double bond in intermediates—cholesta-5,7-dien-3beta-ol (7-dehydrocholesterol/7-DHC) and cholesta-5,7,24-trien-3beta-ol. DHCR7 Protein, Bovine (Cell-Free, His) is the recombinant bovine-derived DHCR7 protein, expressed by E. coli Cell-free, with N-10*His labeled tag. The total length of DHCR7 Protein, Bovine (Cell-Free, His) is 475 a.a., with molecular weight of 55.8 kDa.
研究背景

DHCR7 蛋白，也称为 7-脱氢胆固醇还原酶，是一种参与胆固醇生物合成途径的酶。它在还原该途径中两个中间体的 C7-C8 双键方面发挥着至关重要的作用，即 cholesta-5,7-dien-3beta-ol (7-脱氢胆固醇/7-DHC) 和 cholesta-5,7,24- trien-3beta-ol。
种属

Bovine
表达系统

E. coli Cell-free
标签

N-10*His
蛋白编号

Q5E9J5 (M1-F475)
基因 ID

514745
蛋白结构
N-term
10*His
DHCR7 (M1-F475)
Accession # Q5E9J5
C-term
同用名
7-dehydrocholesterol reductase; Sterol Delta(7)-reductase
氨基酸序列

MAAKSQPSAPKTKSTSGLTNGNAAAQGQWGRAWEVDWFSLASVIFLLLFAPFIVYYFIMACDQYGCSLTVPVADLATGRARLADIWARTPPVTAKAAQIYTAWVTLQVLLYMLLPDFCHKFLPGYVGGVQEGAVTPAGAVNKYEINGLQAWLLTHLLWFANAHLLGWFSPTIIFDNWIPLLWCANILGYTVSTFAMVKGYLFPTDARECKFTGNFFYNYMMGVEFNPRIGKWFDFKLFFNGRPGIVAWTLINLSFAAKQQELYGHVTNSMVLVNILQAIYVLDFFWNETWYLKTIDICHDHFGWYLGWGDCVWLPYLYTLQGLYLVYHPVQLPTYYALGVLLLGLLGYYIFRMTNHQKDLFRRTDGRCLIWGRKPKAIECSYTSADGQRHHSKLLVSGFWGVARHFNYTGDLMGSLAYCLACGGGHLLPYFYIIFMAILLTHRCLRDEHRCANKYGRDWEHYTAAVPYRLLPGIF

分子量

55.8 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

DHCR7 Protein, Bovine (Cell-Free, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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