DR6/TNFRSF21 蛋白, Rat (HEK293, Fc)

DR6/TNFRSF21 protein is the core of apoptosis and may activate NF-kappa-B and promote BAX-mediated apoptosis through cytochrome c release. In neuronal apoptosis, it responds to amyloid peptides in APP, which is critical for cell body death and axonal pruning. DR6/TNFRSF21 Protein, Rat (HEK293, Fc) is the recombinant rat-derived DR6/TNFRSF21 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of DR6/TNFRSF21 Protein, Rat (HEK293, Fc) is 349 a.a., with molecular weight of ~95-115 kDa.

DR6/TNFRSF21 蛋白在促进细胞凋亡中发挥着关键作用，可能是通过涉及 NF-kappa-B 激活的途径实现的。它还可以促进 BAX 介导的细胞凋亡以及细胞色素 c 从线粒体释放到细胞质中。该蛋白积极参与神经元凋亡，对 APP 衍生的淀粉样肽做出反应，对于正常细胞体死亡和轴突修剪至关重要。营养因子剥夺会触发 β 分泌酶裂解表面 APP，释放 sAPP-β，后者与 TNFRSF21 结合，随后激活半胱天冬酶并诱导神经元细胞体（通过 caspase-3）和轴突（通过 caspase-6）退化。除了在神经元过程中的作用外，DR6/TNFRSF21 还负向调节少突胶质细胞的存活、成熟和髓鞘形成。此外，它在 T 细胞受体介导的信号传导、适应性免疫反应以及 T 细胞分化和增殖的调节中发挥作用，对细胞因子释放和抗体产生表现出负调节作用。此外，DR6/TNFRSF21 通过招募 CASP8 来响应活性氧，从而充当细胞焦亡的调节剂，从而导致 GSDMC 的激活。它在其复杂的分子功能网络中与 TRADD、NGFR、CASP8 和 N-APP 相互作用。

Measured by its binding ability in a functional ELISA. When Recombinant Human APP Protein is immobilized at 2 µg/mL (100 µL/well) can bind Recombinant Rat DR6/TNFRSF21 Protein. The ED₅₀ for this effect is 98.85 ng/mL.

Rat

HEK293

C-hFc

D3ZF92 (Q42-H349)

316256  [NCBI]

QPEQKTLSLTGTYRHVDRTTGQVLTCDKCPAGTYVSEHCTNTSLRVCSSCPSGTFTRHENGIERCHDCSQPCPRPMIERLPCAALTDRECICPPGMYQSNGTCAPHTVCPVGWGVRKKGTENEDVRCKQCARGTFSDVPSSVMKCRAHTDCLGQNLMVVKQGTKETDNVCGVHLSSSSTTPSSPGIATFSHPEHTESHDVPSSTYEPQGMNSTDSNSTASVRTKVPSDIQEETVPDNTSSTSGKESTNRTLPNPPQLTHQQGPHHRHILKLLPSSMEATGEKSSTAIKAPKRGHPRQNPHKHFDINEH

Approximately 95-115 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.