1. 重组蛋白
  2. Enzymes & Regulators
  3. Phosphatase
  4. Protein tyrosine phosphatases
  5. Dual Specificity Protein Phosphatase 14 (DUSP14)
  6. DUSP14 蛋白, Human (His-MBP)

DUSP14 蛋白, Human (His-MBP)

目录号: HY-P76307
产品使用指南

DUSP14 蛋白可以使 MAP 激酶失活并使 ERK、JNK 和 p38 MAP 激酶去磷酸化。其负调控延伸至 TCR 信号传导,其中 DUSP14 使 MAP3K7 接头 TAB1 去磷酸化,导致失活。DUSP14 蛋白, Human (His-MBP) 是重组的 DUSP14 蛋白,由 E. coli 表达,带有 N-His, N-MBP 标签。DUSP14 蛋白, Human (His-MBP) 全长 191 个氨基酸,分子量约为 ~60 KDa。

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描述

DUSP14 protein can inactivate MAP kinase and dephosphorylate ERK, JNK and p38 MAP kinase. Its negative regulation extends to TCR signaling, where DUSP14 dephosphorylates the MAP3K7 adapter TAB1, resulting in inactivation. DUSP14 Protein, Human (His-MBP) is the recombinant human-derived DUSP14 protein, expressed by E. coli , with N-His, N-MBP labeled tag. The total length of DUSP14 Protein, Human (His-MBP) is 191 a.a., with molecular weight of ~60 KDa.

研究背景

DUSP14 蛋白在 MAP 激酶失活中发挥着至关重要的作用,对 ERK、JNK 和 p38 MAP 激酶表现出去磷酸化活性。其负调节功能延伸至 TCR 信号传导,其中 DUSP14 使 MAP3K7 接头 TAB1 去磷酸化,导致其失活。这种机制强调了 DUSP14 在调节 TCR 信号通路中的作用,揭示了它参与调节免疫反应。DUSP14 对 MAP 激酶级联关键成分的去磷酸化活性凸显了其微调细胞信号传导的能力,对受 MAP 激酶途径影响的各种生理过程具有潜在影响。

种属

Human

表达系统

E. coli

标签

N-His;N-MBP

蛋白编号

O95147 (M1-H191)

基因 ID
蛋白结构
N-term
His-MBP
DUSP14 (M1-H191)
Accession # O95147
C-term
同用名
Dual specificity protein phosphatase 14; MKP-L; MKP-6; DUSP14
分子量

Approximately 60 kDa.

纯度

Greater than 88% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
DUSP14 Protein, Human (His-MBP)
目录号:
HY-P76307
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