1. 重组蛋白
  2. Viral Proteins
  3. Dengue Virus Proteins
  4. DENV E Proteins
  5. E/Envelope 蛋白, Dengue virus 3 (429a.a, HEK293, His)

E/Envelope 蛋白, Dengue virus 3 (429a.a, HEK293, His)

目录号: HY-P74192
产品使用指南

NS1 蛋白在病毒出芽、与细胞膜结合以及促进病毒 RNA 组装成核衣壳以形成成熟病毒颗粒方面发挥着关键作用。在进入过程中,NS1 诱导基因组渗透到宿主细胞质中。E/Envelope 蛋白, Dengue virus 3 (429a.a, HEK293, His) 是重组的 E/包膜蛋白,由 HEK293 表达,带有 C-His 标签。E/Envelope 蛋白, Dengue virus 3 (429a.a, HEK293, His) 全长 429 个氨基酸,分子量约为 ~48.79 kDa。

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描述

The NS1 protein plays a key role in viral budding, association with cell membranes, and facilitating the assembly of viral RNA into nucleocapsids to form mature viral particles. During entry, NS1 induces genome penetration into the host cytoplasm. E/Envelope Protein, Dengue virus 3 (429a.a, HEK293, His) is the recombinant Virus-derived E/Envelope protein, expressed by HEK293 , with C-His labeled tag. The total length of E/Envelope Protein, Dengue virus 3 (429a.a, HEK293, His) is 429 a.a., with molecular weight of ~48.79 kDa.

研究背景

NS1 蛋白通过与细胞膜结合,促进病毒 RNA 组装成核衣壳,从而形成成熟病毒颗粒的核心,从而在病毒出芽中发挥关键作用。在病毒进入过程中,NS1 可能会在表面蛋白诱导的半融合后诱导基因组渗透到宿主细胞质中。值得注意的是,NS1 表现出迁移到细胞核的能力,在细胞核中调节宿主功能。此外,NS1 通过蛋白酶体降解途径隔离和降解 EXOC1,抵消宿主 EXOC1 的抗病毒作用。此外,NS1 通过干扰宿主 Dicer 来破坏 RNA 沉默,突显其在整个感染周期中操纵病毒和宿主细胞过程中的多方面作用。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

AAA99437 (F247-S675)

基因 ID

80527852

蛋白结构
N-term
DENV3 E (F247-S675)
Accession # AAA99437
His
C-term
同用名
E Protein; DENV; Dengue virus (DENV)(type 3, strain Philippines/H87/1956) E / Envelope Protein (aa 247-675, ECD, His Tag)
分子量

Approximately 48.79 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

E/Envelope Protein, Dengue virus 3 (429a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
E/Envelope Protein, Dengue virus 3 (429a.a, HEK293, His)
目录号:
HY-P74192
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