1. 重组蛋白
  2. Viral Proteins
  3. Ebola Virus Proteins
  4. Ebola Virus GP Proteins
  5. Ebola virus Glycoprotein/GP 蛋白 (AIE11917, HEK293, His)

Ebola virus Glycoprotein/GP 蛋白 (AIE11917, HEK293, His)

目录号: HY-P75272
COA 产品使用指南

埃博拉病毒糖蛋白(GP)在病毒进入过程中发挥着关键作用,其中GP1作为受体结合亚基,GP2用于膜融合。GP 下调宿主细胞表面分子,破坏粘附,并可能导致血管完整性破坏。Ebola virus Glycoprotein/GP 蛋白 (AIE11917, HEK293, His) 是重组的 埃博拉病毒糖蛋白/GP蛋白,由 HEK293 表达,带有 C-His 标签。Ebola virus Glycoprotein/GP 蛋白 (AIE11917, HEK293, His) 全长 618 个氨基酸,分子量约为 ~69.3 kDa。

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描述

Ebola virus glycoproteins (GPs) play a key role in the virus entry process, with GP1 serving as the receptor-binding subunit and GP2 for membrane fusion. GP downregulates host cell surface molecules, disrupts adhesion and may lead to disruption of vascular integrity. Ebola virus Glycoprotein/GP Protein (AIE11917, HEK293, His) is the recombinant Virus-derived Ebola virus Glycoprotein/GP protein, expressed by HEK293 , with C-His labeled tag. The total length of Ebola virus Glycoprotein/GP Protein (AIE11917, HEK293, His) is 618 a.a., with molecular weight of ~69.3 kDa.

研究背景

埃博拉病毒糖蛋白 (GP) 的三聚体 GP1,2 复合物在病毒进入过程中发挥着至关重要的作用,其中 GP1 充当受体结合亚基,GP2 充当膜融合亚基。在感染后期,GP下调各种宿主细胞表面分子的表达,包括整合素,如ITGA1、ITGA2、ITGA3、ITGA4、ITGA5、ITGA6、ITGAV和ITGB1,破坏细胞粘附并导致细胞脱离,可能导致血管完整性破坏和出血。GP 还与宿主 TLR4 相互作用,刺激单核细胞的分化和激活,导致 T 淋巴细胞的旁观者死亡。此外,GP可以抵消宿主BST2/tetherin的抗病毒作用,与VP40和宿主BST2合作激活经典的NF-kappa-B通路,并作为抗GP1,2抗体的诱饵,有助于病毒免疫逃避。此外,GP 与宿主巨噬细胞和树突状细胞相互作用并激活它们,通过激活宿主 TLR4 诱导细胞因子转录上调。

种属

Virus

表达系统

HEK293

标签

C-His

蛋白编号

AIE11917 (I33-Q650)

基因 ID

/

蛋白结构
N-term
EBOV GP (I33-Q650)
Accession # AIE11917
His
C-term
同用名
Ebola virus EBOV (subtype Zaire, strain Ebola virus H.sapiens-wt/SLE/2014/ManoRiver-G3686.1) Glycoprotein / EBOV-G Protein (His)
氨基酸序列

IPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSVTKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGFPRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVAFLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEYLFEVDNLTYVQLESRFTPQFLLQLNETIYASGKRSNTTGKLIWKVNPEIDTTIGEWAFWETKKNLTRKIRSEELSFTAVSNGPKNISGQSPARTSSDPETNTTNEDHKIMASENSSAMVQVHSQGRKAAVSHLTTLATISTSPQPPTTKTGPDNSTHNTPVYKLDISEATQVGQHHRRADNDSTASDTPPATTAAGPLKAENTNTSKSADSLDLATTTSPQNYSETAGNNNTHHQDTGEESASSGKLGLITNTIAGVAGLITGGRRTRREVIVNAQPKCNPNLHYWTTQDEGAAIGLAWIPYFGPAAEGIYTEGLMHNQDGLICGLRQLANETTQALQLFLRATTELRTFSILNRKAIDFLLQRWGGTCHILGPDCCIEPHDWTKNITDKIDQIIHDFVDKTLPDQGDNDNWWTGWRQ

分子量

Approximately 69.3 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose). 组成 Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Ebola virus Glycoprotein/GP Protein (AIE11917, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Ebola virus Glycoprotein/GP Protein (AIE11917, HEK293, His)
目录号:
HY-P75272
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