Ebola virus NP/Nucleoprotein 蛋白 (109a.a, Q5XX08, His)

The Ebola virus NP/nucleoprotein is critical for viral genome protection and replication, forming a helical capsid to protect the genome. The VP35 interaction stabilizes the monomeric NP, maintaining solubility until replication triggers cooperative binding to viral RNA. Ebola virus NP/Nucleoprotein Protein (109a.a, Q5XX08, His) is the recombinant Virus-derived Ebola virus NP/Nucleoprotein protein, expressed by E. coli , with N-His labeled tag. The total length of Ebola virus NP/Nucleoprotein Protein (109a.a, Q5XX08, His) is 109 a.a., with molecular weight of ~14 kDa.

埃博拉病毒 NP/核蛋白通过寡聚成包裹病毒基因组的螺旋衣壳，保护其免受细胞核酸酶和先天免疫反应的影响，在病毒基因组保护和复制中发挥关键作用。与 VP35 的相互作用可稳定单体 NP，保持其溶解度，直到病毒复制触发 NP 与病毒基因组 RNA 的协同结合，从而导致 VP35 的释放。这种衣壳化的基因组 RNA 形成核衣壳，作为转录和复制的模板，具有螺旋结构，每圈螺距为 10.81 NP，直径约为 22 nm。NP 与病毒基因组 RNA 的 6 个核苷酸结合，其中 3 个暴露于溶剂，另外 3 个隐藏在核衣壳内。此外，NP 招募宿主 PPP2R5C 磷酸酶来使 VP30 去磷酸化，从而促进病毒转录。在病毒粒子组装过程中，NP 与 VP24 和潜在的宿主 STAU1 相互作用，促进核衣壳组装和基因组包装。此外，与 VP40、宿主 NXF1 和 CCDC92 的相互作用进一步促进了 NP 在埃博拉病毒生命周期中的多方面功能。

Data is not available.

Virus

E. coli

N-His

Q5XX08 (G630-D738)

3160777  [NCBI]

GQGSESEALPINPEKGSALEETYYHLLKTQGPFEAINYYHLMSDEPIAFSTESGKEYIFPDSLEEAYPPWLSEKEALEKENRYLVIDGQQFLWPVMSLQDKFLAVLQHD

Approximately 14 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.