2. 重组蛋白
  3. Viral Proteins
  4. Ebola Virus Proteins
  5. Ebola Virus VP40
  6. Ebola virus VP40/Matrix VP40 蛋白 (B8XCM9, His)

Ebola virus VP40/Matrix VP40 蛋白 (B8XCM9, His)

目录号: HY-P75716
产品使用指南

埃博拉病毒 VP40/基质 VP40 蛋白对于病毒颗粒的组装和萌芽、与与多泡体途径相关的宿主蛋白相互作用至关重要。Ebola virus VP40/Matrix VP40 蛋白 (B8XCM9, His) 是重组的 埃博拉病毒 VP40/基质 VP40 蛋白，由 E. coli 表达，带有 N-His 标签。Ebola virus VP40/Matrix VP40 蛋白 (B8XCM9, His) 全长 296 个氨基酸，分子量约为 ~34.4 kDa。

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描述

Ebola virus VP40/Matrix VP40 Protein is essential for assembling and budding virus particles, interacting with host proteins linked to the multivesicular body pathway. Ebola virus VP40/Matrix VP40 Protein (B8XCM9, His) is the recombinant Virus-derived Ebola virus VP40/Matrix VP40 protein, expressed by E. coli , with N-His labeled tag. The total length of Ebola virus VP40/Matrix VP40 Protein (B8XCM9, His) is 296 a.a., with molecular weight of ~34.4 kDa.
研究背景

埃博拉病毒 VP40/Matrix VP40 蛋白对于病毒颗粒的组装和出芽至关重要。它通过与多泡体途径中涉及的宿主蛋白相互作用来促进病毒组装和出芽。
种属

Virus
表达系统

E. coli
标签

N-His
蛋白编号

B8XCM9 (A31-K326)
基因 ID

9487264  [NCBI]
蛋白结构
N-term
His
EBOV VP40 (A31-K326)
Accession # B8XCM9
C-term
同用名
Ebola virus EBOV (subtype Bundibugyo, strain Uganda 2007) VP40 / Matrix protein VP40 Protein (His)
分子量

Approximately 34.4 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Ebola virus VP40/Matrix VP40 Protein (B8XCM9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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