2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. ECE-1 蛋白, Human (HEK293, His)

ECE-1 蛋白, Human (HEK293, His)

目录号: HY-P70035
产品使用指南

ECE-1 蛋白通过催化大内皮素-1 转化为内皮素-1，在血管调节中发挥关键作用。这一酶促过程是内皮素-1 成熟的关键步骤，内皮素-1 是一种有效的血管收缩剂和信号分子，参与血压、血管张力和各种生理功能的调节。ECE-1 蛋白, Human (HEK293, His) 是重组的 ECE-1 蛋白，由 HEK293 表达，带有 N-8*His 标签。ECE-1 蛋白, Human (HEK293, His) 全长 681 个氨基酸，分子量为 94-130 kDa。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The ECE-1 protein plays a key role in vascular regulation by catalyzing the conversion of large endothelin-1 to endothelin-1. This enzymatic process is a critical step in the maturation of endothelin-1, a potent vasoconstrictor and signaling molecule involved in the regulation of blood pressure, vascular tone, and various physiological functions. ECE-1 Protein, Human (HEK293, His) is the recombinant human-derived ECE-1 protein, expressed by HEK293 , with N-8*His labeled tag. The total length of ECE-1 Protein, Human (HEK293, His) is 681 a.a., with molecular weight of 94-130 kDa.
研究背景

ECE-1 蛋白是参与大内皮素-1 向内皮素-1 转化的关键酶。这种转化对于内皮素-1 的成熟和激活起着关键作用，内皮素-1 是一种具有有效血管收缩作用的肽。通过催化大内皮素-1 的裂解，ECE-1 有助于调节血管张力和血压。内皮素-1 的激活对多种生理过程有影响，包括心血管稳态和平滑肌收缩。作为内皮素系统中的关键酶，ECE-1 的酶活性对于调节血管功能和维持心血管健康至关重要（参考：未提供）。
种属

Human
表达系统

HEK293
标签

N-8*His
蛋白编号

P42892 (Q90-W770)
基因 ID
蛋白结构
N-term
8*His
ECE-1 (Q90-W770)
Accession # P42892
C-term
同用名
rHuEndothelin-converting enzyme 1/ECE-1, His; Endothelin-converting enzyme 1; ECE-1
氨基酸序列

QYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWEHNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDNFQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNYMVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWLPFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDADEKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESLSTLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRVTADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHELTHAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGGLKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRVIGSLSNSKEFSEHFRCPPGSPMNPPHKCEVW

分子量

94-130 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

ECE-1 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
ECE-1 Protein, Human (HEK293, His)
目录号：
HY-P70035
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z