EFNB2A 蛋白, zebrafish (HEK293, His)

The EFNB2A protein is a cell surface ligand of the Eph receptor that critically regulates migration, repulsion, and adhesion in neuronal, vascular, and epithelial development. EFNB2A Protein, zebrafish (HEK293, His) is the recombinant EFNB2A protein, expressed by HEK293 , with C-His labeled tag. The total length of EFNB2A Protein, zebrafish (HEK293, His) is 198 a.a., with molecular weight of ~30-40 kDa.

EFNB2A 是 Eph 受体的细胞表面跨膜配体，通过与相邻细胞上的 Eph 受体混杂结合，在神经元、血管和上皮发育中发挥着至关重要的作用。这种相互作用启动接触依赖性双向信号传导至邻近细胞，其中正向信号发生在受体下游，反向信号发生在肝配蛋白配体下游。EFNB2A 与 EphB4 一起可能通过调节细胞粘附和迁移在心脏形态发生和血管生成中发挥重要作用。该蛋白与受体酪氨酸激酶 EphB4 的结合凸显了它参与这些重要的发育过程。

Immobilized zebrafish EFNB2A at 10 µg/mL (100 µL/well) can bind Biotinylated human EphB4. The ED₅₀ for this effect is 31.44 ng/mL.

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  Immobilized zebrafish EFNB2A at 10 µg/mL (100 µL/well) can bind Biotinylated human EphB4. The ED₅₀ for this effect is 31.44 ng/mL.

Others

HEK293

C-His

O73874 (L25-A222)

30219  [NCBI]

LILDSIYWNTTNTKFVPGQGLVLYPQIGDKMDIVCPRVEGGSMEGVEYYKLYMVPLEQLKSCQVTKADTPLLNCVKPDQDVKFTLKFQEFSPNLWGLEFFRGKDYYIISTSNGTMEGLDNQEGGVCKTKSMKIIMKVGQNPSDPISPKDYPTSYPPKHPDLGGKDSKSNEVLKPDASPHGEDKGDGNKSSSVIGSEVA

Approximately 30-40 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, PH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.