1. 重组蛋白
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. EGF
  5. EGF 蛋白, Rat

EGF 蛋白, Rat

目录号: HY-P7092
COA 产品使用指南

EGF 蛋白, Rat是一种有效的表皮生长因子,能够促进上皮细胞增殖和迁移,能够加快表皮伤口愈合,缩短治疗时间。

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2 μg ¥350 In-stock
10 μg ¥800 询价
50 μg ¥1400 In-stock
100 μg ¥2100 询价
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

EGF Protein, Rat is a potent epidermal growth factor, promotes epithelial proliferation and migration, and increases epithelial wound closure and shortens healing time.

研究背景

表皮生长因子由上皮细胞分泌,促进多种组织中的上皮增殖和迁移,促进上皮伤口闭合,缩短愈合时间[1]。表皮生长因子在皮肤发育和体内平衡中发挥着关键作用,在表皮屏障中显示出保护功能。表皮生长因子还在发炎的皮肤组织中显示出免疫调节活性[2]

生物活性

Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.05-0.37 ng/mL.

  • Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 this effect is 199.3 pg/mL, corresponding to a specific activity is 5.02×106 U/mg.
种属

Rat

表达系统

E. coli

标签

Tag Free

蛋白编号

P07522/NP_036974.1 (N974-R1026)

基因 ID

25313  [NCBI]

蛋白结构
N-term
EGF (N974-R1026)
Accession # P07522
C-term
中文名
重组大鼠表皮生长因子
同用名
rRtEGF; Pro-epidermal growth factor; Urogastrone
氨基酸序列

NSNTGCPPSYDGYCLNGGVCMYVESVDRYVCNCVIGYIGERCQHRDLRWWKLR

分子量

Approximately 10.42 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

EGF Protein, Rat 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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