EIF5A2 蛋白, Human (His)

EIF5A2 is a translation factor critical for promoting elongation and termination, helping stalled ribosomes encounter specific amino acid environments. EIF5A2 is located between the ribosome exit (E) and peptidyl (P) sites and rescues stalled ribosomes, especially those translating polyproline-containing peptides. EIF5A2 Protein, Human (His) is the recombinant human-derived EIF5A2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of EIF5A2 Protein, Human (His) is 153 a.a., with molecular weight of ~22.0 kDa.

EIF5A2 是一种翻译因子，在促进翻译延伸和终止方面发挥着关键作用，尤其是当核糖体遇到特定氨基酸序列背景导致翻译停滞时。EIF5A2 位于核糖体的出口 (E) 和肽基 (P) 位点之间，有助于拯救停滞的核糖体，特别是那些翻译含聚脯氨酸的肽和其他导致核糖体停滞的基序的核糖体。此外，EIF5A2 还充当核糖体质量控制 (RQC) 中的辅助因子，在 CAT 加尾步骤期间帮助 RQC 复合物进行肽基转移。除了在翻译中的作用之外，EIF5A2 还与肌动蛋白动力学、细胞周期进程、mRNA 衰减有关，并且可能参与应激反应和细胞壁完整性维护。它在主动翻译核糖体上与 EEF2/eEF2 表现出相互排斥的结合。值得注意的是，EIF5A2 与 DAPL1 相互作用，在冬眠核糖体的多肽出口通道处形成复合物，从而阻止翻译。

Human

E. coli

N-6*His

Q9GZV4 (M1-K153)

56648  [NCBI]

MADEIDFTTGDAGASSTYPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDYQLICIQDGYLSLLTETGEVREDLKLPEGELGKEIEGKYNAGEDVQVSVMCAMSEEYAVAIKPCK

Approximately 22.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.