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ELAVL4 蛋白, Human (His-SUMO)

目录号: HY-P72180
COA 产品使用指南

ELAVL4 蛋白是一种广泛的 RNA 结合因子,可在转录后调节 GAP43、VEGF、FOS、CDKN1A 和 ACHE 等 mRNA,通过与 ARE 序列结合来稳定并保护它们免于衰变。它通过与 Poly(A) 尾相互作用减少 mRNA 去腺苷化并增强 mRNA 结合亲和力。ELAVL4 蛋白, Human (His-SUMO) 是重组的 ELAVL4 蛋白,由 E. coli 表达,带有 N-10*His, N-SUMO, C-Myc 标签。ELAVL4 蛋白, Human (His-SUMO) 全长 380 个氨基酸,分子量约为 ~61.8 kDa。

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描述

ELAVL4 protein is a broad RNA-binding factor that post-transcriptionally regulates mRNAs such as GAP43, VEGF, FOS, CDKN1A, and ACHE, stabilizing and protecting them from decay by binding to ARE sequences. It reduces mRNA deadenylation and enhances mRNA binding affinity by interacting with the Poly(A) tail. ELAVL4 Protein, Human (His-SUMO) is the recombinant human-derived ELAVL4 protein, expressed by E. coli , with N-10*His, N-SUMO, C-Myc labeled tag. The total length of ELAVL4 Protein, Human (His-SUMO) is 380 a.a., with molecular weight of ~61.8 kDa.

研究背景

ELAVL4 蛋白是一种 RNA 结合因子,广泛参与 mRNA 的转录后调节,影响 mRNA 的稳定性、选择性剪接和翻译。通过与目标 mRNA(包括 GAP43、VEGF、FOS、CDKN1A 和 ACHE)3' 非翻译区 (UTR) 中富含 AU 的元件 (ARE) 序列结合,ELAVL4 有助于稳定和保护这些转录本免遭衰变。它通过与 mRNA 3'UTR 结合来减少 mRNA 去腺苷化,并且还与 3'UTR 中的聚腺苷酸 (poly(A)) 尾部相互作用,从而增强 mRNA 结合亲和力。ELAVL4 主要在神经元特异性 RNA 加工中发挥作用,稳定与神经元蛋白相关的 mRNA,并有助于神经祖细胞分化、神经系统发育以及学习和记忆机制。此外,它还在神经元干细胞增殖活性的负调节中发挥作用,促进神经元分化。ELAVL4 的影响延伸到神经突生长、树突乔木的建立和成熟,影响新皮质和海马回路功能。它形成 TAU mRNP 复合物的一部分,与 EIF4F 帽结合复合物结合,并与各种蛋白质相互作用,包括 SMN、MAP1 轻链 LC1 和 LC2,突出了其在 RNA 调节和神经元过程中的多种作用。

种属

Human

表达系统

E. coli

标签

N-10*His;N-SUMO;C-Myc

蛋白编号

P26378 (M1-S380)

基因 ID
蛋白结构
N-term
10*His
ELAVL4 (M1-S380)
Accession # P26378
Myc
C-term
同用名
ELAV embryonic lethal abnormal vision Drosophila; like 4; ELAV L4; ELAV like 4; ELAV like protein 4; ELAV-like protein 4; ELAV4_HUMAN; Elavl4; Embryonic lethal abnormal vision Drosophila homolog of like 4; Hu antigen D; Hu-antigen D; HuD; Paraneoplastic encephalomyelitis antigen HuD; PNEM
氨基酸序列

MVMIISTMEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSKTNLIVNYLPQNMTQEEFRSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPSGATEPITVKFANNPSQKSSQALLSQLYQSPNRRYPGPLHHQAQRFRLDNLLNMAYGVKRLMSGPVPPSACPPRFSPITIDGMTSLVGMNIPGHTGTGWCIFVYNLSPDSDESVLWQLFGPFGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKAHKS

分子量

Approximately 61.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

ELAVL4 Protein, Human (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ELAVL4 Protein, Human (His-SUMO)
目录号:
HY-P72180
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