1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Chemokine & Receptors
  4. CXC Chemokines
  5. ENA-78
  6. ENA-78/CXCL5 蛋白, Human (HEK293)

ENA-78/CXCL5 蛋白, Human (HEK293)

目录号: HY-P7157
COA 产品使用指南

CXCL5,也称为中性粒细胞激活肽 78 (ENA-78),是一种含有 ELR 基序的CXC 趋化因子。CXCL5 通过与其特异性受体 CXCR2 相互作用促进血管生成。CXCL5 由许多免疫细胞表达,例如巨噬细胞、嗜酸性粒细胞和包括间皮细胞和成纤维细胞在内的非免疫细胞。CXCL5/CXCR2 信号轴不仅有助于中性粒细胞的募集,而且还调节黑色素瘤中中性粒细胞的功能。ENA-78/CXCL5 蛋白, Human (HEK293) 在 HEK293 细胞中表达产生,由 78 个氨基酸(A37-N114) 组成。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥3600 In-stock
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描述

CXCL5, also known as neutrophil activating peptide 78 (ENA‐78), is a CXC chemokine containing ELR motif. CXCL5 promotes angiogenesis through interaction with its specific receptor CXCR2. CXCL5 is expressed by many immune cells, such as macrophages, eosinophils, and non-immune cells including mesothelial cells, and fibroblasts.CXCL5/CXCR2 axis not only contributes to the recruitment of neutrophils but also regulates the function of neutrophils in melanoma[1][2]. ENA-78/CXCL5 Protein, Human (HEK293) is produced in HEK293 cells, and consists of 78 amino acids (A37-N114).

研究背景

CXCL5 belongs to the CXC‐type chemokine family with a specific amino acid sequence of glutamic acid-leucine-arginine which is shortly named as ELR motif. The ELR motif of CXC‐type chemokine family plays an important role in angiogenesis. CXCL5 is expressed by many immune cells, such as macrophages, eosinophils, as well as non-immune cells including mesothelial cells, and fibroblasts[1][2].
Human CXCL5 shares 57% amino acid sequence identity with mouse and rat CXCL5.
CXCL5 can recruit cells, such as T/B lymphocytes and eosinophils from the immune system to the corresponding regions during immune response. Besides, it also participates in promoting the adhesion and remodeling of connective tissues. CXCL5 is highly expressed in various tumor tissues. CXCL5 can be used as a potential indicator of tumor prognosis. In tumor microenvironment (TME), CXCL5 binds to its receptors, such as CXCR2, to participate in the recruitment of immune cells and promote angiogenesis, tumor growth, and metastasis. Mesenchymal stem cells (MSCs) activated by acidic TME or TNF‐α can secrete CXCL5 and other pro‐inflammatory factors. Besides, cancer‐associated mesothelial cells, which are generated by plasminogen activator inhibitor‐1 (PAI‐1), can secrete IL‐8 and CXCL5 via the NF-κB signaling pathway and thereby promoting peritoneal metastasis. Adipose tissue‐derived stem cells (ASCs) secrete CXCL5 which subsequently promotes tumor growth and affects the development of breast tumors[2].
The CXCL5/CXCR2 axis recruits neutrophils, promotes angiogenesis and remodels connective tissues. In addition, CXCL5 promotes angiogenesis via activating the AKT/NF-κB/FOXD1/VEGF-A pathway in a CXCR2-dependent manner. CXCL5 plays a role in cancer cell proliferation, migration, and invasion. CXCL5 promotes the proliferation of several types of tumor cells, such as the tumor cells in prostate cancer, cervical cancer, lung cancer, hepatoblastoma, and osteosarcoma[1][2][3].

体内研究
(In Vivo)

Recombinant human CXCL5 protein (1 ng/mL; 24-48 h) promotes proliferation, migration and partial invasion in Caco-2, HT-29 and SW-480 cells[1].
Recombinant human CXCL5 protein (1-10 ng/mL; 36 h) stimulates the formation ability, proliferation, and migration in HUVECs, which are enhanced by the activation of the AKT/NF-κB/FOXD1/VEGF-A pathway in a CXCR2-dependent manner[3].

生物活性

The ED50 is <200 ng/mL as measured by CHO-K1/Gα15/hCXCR2 cells (human Gα15 and human CXCR2 stably expressed in CHO-K1 cells).

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P42830 (A37-N114)

基因 ID

6374  [NCBI]

中文名
重组人 CXC 趋化因子 5 (HEK293)
同用名
rHuENA-78/CXCL5; ENA78; SCYB5
氨基酸序列

AGPAAAVLRELRCVCLQTTQGVHPKMISNLQVFAIGPQCSKVEVVASLKNGKEICLDPEAPFLKKVIQKILDGGNKEN

分子量

Approximately 8.5 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

ENA-78/CXCL5 Protein, Human (HEK293) 相关分类

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ENA-78/CXCL5 Protein, Human (HEK293)
目录号:
HY-P7157
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