ENPP-1 蛋白, Human (Biotinylated, HEK293, His-Avi)

ENPP-1 protein is a nucleotide pyrophosphatase that inhibits excessive hydroxyapatite crystal growth by preferentially hydrolyzing ATP and other nucleoside triphosphates to generate PPi, thereby critically regulating bone mineralization and soft tissue calcification. ENPP-1 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived ENPP-1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of ENPP-1 Protein, Human (Biotinylated, HEK293, His-Avi) is 828 a.a., with molecular weight of 110-120 kDa.

ENPP-1 蛋白是一种核苷酸焦磷酸酶，通过调节焦磷酸盐水平在骨矿化和软组织钙化中发挥至关重要的作用。ENPP-1通过优先水解ATP和其他核苷5'三磷酸，如GTP、CTP和UTP，生成二磷酸（PPi），抑制新生羟基磷灰石晶体的进一步生长，从而防止骨过度矿化和软组织钙化。此外，它还参与二腺苷多磷酸和 3',5'-cAMP 水解为 AMP，并可能调节内质网和高尔基体中核苷酸糖的可用性，影响嘌呤能信号传导。ENPP-1 还有助于抑制刺猬信号传导、抑制异位关节钙化并维持关节软骨细胞。此外，它还参与调节胰岛素敏感性和功能、参与黑素生成，并且可以水解 2',3'-cGAMP（参与触发 I 型干扰素产生的第二信使）。2',3'-cGAMP 水解在细胞外空间中的确切作用仍不清楚。

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Immobilized Biotinylated Human ENPP-1, His Tag at 0.5 μg/mL (100 μl/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC₅₀ of 4.5 ng/mL determined by ELISA.

Human

HEK293

C-Avi;C-His

P22413 (K98-D925)

5167  [NCBI]

110-120 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in 20 mM Tris,150 mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.