ENPP-3 蛋白, Human (828a.a, HEK293, hFc)

ENPP3 is a hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP, and CTP, and plays a key role in immune regulation. It modulates mast cell and basophil responses during inflammation and chronic allergic phases by eliminating extracellular ATP, a trigger for inflammatory cytokine release. ENPP-3 Protein, Human (828a.a, HEK293, hFc) is the recombinant human-derived ENPP-3 protein, expressed by HEK293, with N-hFc labeled tag.

ENPP3 是一种水解酶，在细胞外核苷酸（包括 ATP、GTP、UTP 和 CTP）代谢中发挥着关键作用。这种酶活性有助于调节免疫反应，特别是在炎症和慢性过敏阶段调节肥大细胞和嗜碱性粒细胞反应。ENPP3 通过消除细胞外 ATP（一种激活嗜碱性粒细胞和肥大细胞的信号分子，随后触发炎症细胞因子的释放）来实现这一目标。此外，在小肠腔内，ENPP3 代谢细胞外 ATP，有效防止 ATP 诱导的肠浆细胞样树突状细胞凋亡。除了参与核苷酸代谢外，ENPP3 还表现出碱性磷酸二酯酶活性，增加了其在细胞过程中的多种功能。

Human

HEK293

N-hFc

O14638 (L48-I875)

5169

150-190 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.