ENPP-2 蛋白, Mouse (HEK293, His)

ENPP-2 Protein catalyzes the hydrolysis of lysophospholipids, particularly lysophosphatidylcholine, generating the signaling molecule lysophosphatidic acid (LPA). It also acts on sphingosylphosphorylcholine, producing sphingosine-1-phosphate, influencing cell motility. ENPP-2 is involved in angiogenesis, neurite outgrowth, and tumor cell motility, showing a preference for specific lysophospholipid isomers. It plays a role in platelet activation, contributing to LPA production and cleaving sn-1 lysophospholipids. ENPP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-2 protein, expressed by HEK293 , with N-His, N-10*His labeled tag. The total length of ENPP-2 Protein, Mouse (HEK293, His) is 814 a.a., with molecular weight of ~100 kDa.

ENPP-2 蛋白水解溶血磷脂，特别是溶血磷脂酰胆碱，在细胞外液中产生信号分子溶血磷脂酸 (LPA)。它还可以作用于鞘氨醇磷酸胆碱，产生 1-磷酸鞘氨醇，调节细胞运动。此外，ENPP-2 蛋白已被证明可以不同程度地水解 bis-pNPP、pNP-TMP 和 ATP。它参与各种运动相关过程，包括血管生成和神经突生长，并通过刺激平滑肌细胞的迁移和微管形成而充当血管生成因子。 ENPP-2 蛋白还可能通过百日咳毒素敏感的 G 蛋白参与黑色素瘤细胞迁移。它可能在诱导分娩、细胞增殖和脂肪组织发育中发挥作用。此外，活化血小板中 LPA 的产生以及裂解 sn-1 溶血磷脂以产生主要含有 18:2 和 20:4 脂肪酸的 sn-1 溶血磷脂酸是必需的。 ENPP-2 蛋白优先选择 1-O-烷基-sn-甘油-3-磷酸胆碱 (lyso-PAF) 的 sn-1 异构体。

Mouse

HEK293

N-His;N-10*His

Q9R1E6-1 (S49-I862)

18606  [NCBI]

SPWTNTSGSCKGRCFELQEVGPPDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGESHWVDDDCEEIRVPECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVRAGTFFWSVSIPHERRILTILQWLSLPDNERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRPKIPNNLKYDPKAIIANLTCKKPDQHFKPYMKQHLPKRLHYANNRRIEDLHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGPTFKYRTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTLPEEVSRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLEELNKRLHTKGSTEERHLLYGRPAVLYRTSYDILYHTDFESGYSEIFLMPLWTSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWTYFQRVLVKKYASERNGVNVISGPIFDYNYNGLRDIEDEIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNDESCNSSEDESKWVEELMKMHTARVRDIEHLTGLDFYRKTSRSYSEILTLKTYLHTYESEI

Approximately 100 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.