ENPP3 蛋白, Human (HEK293, His)

ENPP3 is a hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP, and CTP, and plays a key role in immune regulation. It modulates mast cell and basophil responses during inflammation and chronic allergic phases by eliminating extracellular ATP, a trigger for inflammatory cytokine release. ENPP3 Protein, Human (HEK293, His) is the recombinant human-derived ENPP3 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of ENPP3 Protein, Human (HEK293, His) is 828 a.a., with molecular weight of ~116 kDa.

ENPP3 是一种水解酶，在细胞外核苷酸（包括 ATP、GTP、UTP 和 CTP）代谢中发挥着关键作用。这种酶活性有助于调节免疫反应，特别是在炎症和慢性过敏阶段调节肥大细胞和嗜碱性粒细胞反应。ENPP3 通过消除细胞外 ATP（一种激活嗜碱性粒细胞和肥大细胞的信号分子，随后触发炎症细胞因子的释放）来实现这一目标。此外，在小肠腔内，ENPP3 代谢细胞外 ATP，有效防止 ATP 诱导的肠浆细胞样树突状细胞凋亡。除了参与核苷酸代谢外，ENPP3 还表现出碱性磷酸二酯酶活性，增加了其在细胞过程中的多种功能。

Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3 at 2 μg/mL can bind anti-ENPP3 recombinant antibody, the EC₅₀ is ≤ 2.868 ng/mL.

Human

HEK293

C-10*His

O14638 (L48-I875)

5169  [NCBI]

LEKQGSCRKKCFDASFRGLENCRCDVACKDRGDCCWDFEDTCVESTRIWMCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFFYMYEGPAPRIRAHNIPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDQQWLAVRSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTHGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI

Approximately 116 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.