ENPP-7 蛋白, Human (HEK293, His)

The ENPP-7 protein is a choline-specific phosphodiesterase that plays multiple roles in sphingomyelin digestion, ceramide formation, and fatty acid absorption in the gastrointestinal tract. It hydrolyzes sphingomyelin, releases ceramide and phosphocholine, and promotes the breakdown of sphingomyelin. ENPP-7 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-7 protein, expressed by HEK293 , with C-His labeled tag. The total length of ENPP-7 Protein, Human (HEK293, His) is 418 a.a., with molecular weight of ~50-70 kDa.

ENPP-7 蛋白作为胆碱特异性磷酸二酯酶发挥作用，在胃肠道内的鞘磷脂消化、神经酰胺形成和脂肪酸 (FA) 吸收中发挥多方面作用。值得注意的是，它会水解鞘磷脂，释放神经酰胺和磷酸胆碱，从而参与鞘磷脂分解和随后神经酰胺产生的复杂过程。此外，ENPP-7 还表现出磷脂酶 C 活性，将磷酸胆碱从棕榈酰溶血磷脂酰胆碱和血小板激活因子 (PAF) 中裂解出来，从而导致它们失活。尽管缺乏核苷酸焦磷酸酶活性，ENPP-7 可能通过影响肠腔内膳食和内源性鞘磷脂的水平来促进胆固醇吸收。这些不同的酶活性凸显了 ENPP-7 在脂质代谢和吸收过程中的多功能作用，强调了其在胃肠道生理学中的重要性。

Measured by its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine. The phosphorylcholine is cleaved by Recombinant Human Alkaline Phosphatase/ALPL and the phosphate is measured by a Malachite Green Phosphate Detection Kit. The specific activity is 14449.85 pmoL/min/μg.

Human

HEK293

C-His

Q6UWV6 (A22-S439)

339221  [NCBI]

APVQSQGSQNKLLLVSFDGFRWNYDQDVDTPNLDAMARDGVKARYMTPAFVTMTSPCHFTLVTGKYIENHGVVHNMYYNTTSKVKLPYHATLGIQRWWDNGSVPIWITAQRQGLRAGSFFYPGGNVTYQGVAVTRSRKEGIAHNYKNETEWRANIDTVMAWFTEEDLDLVTLYFGEPDSTGHRYGPESPERREMVRQVDRTVGYLRESIARNHLTDRLNLIITSDHGMTTVDKRAGDLVEFHKFPNFTFRDIEFELLDYGPNGMLLPKEGRLEKVYDALKDAHPKLHVYKKEAFPEAFHYANNPRVTPLLMYSDLGYVIHGRINVQFNNGEHGFDNKDMDMKTIFRAVGPSFRAGLEVEPFESVHVYELMCRLLGIVPEANDGHLATLLPMLHTESALPPDGRPTLLPKGRSALPPSS

Approximately 50-70 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.