Enterokinase 蛋白, Bovine (P.pastoris, His)

Enterokinase Protein, Bovine (P.pastoris, His) is a glycosylated peptide chain with a His-tag produced in P. pastoris. Enterokinase Protein is a serine protease that plays a crucial role in the digestive system. By specifically cleaving trypsinogen to convert it into active trypsin, Enterokinase Protein initiates the cascade activation of pancreatic zymogens. Enterokinase Protein serves as a key initiator of the intestinal digestive enzyme cascade^[1][2][3].

Enterokinase 是一种糖蛋白，主要存在于十二指肠和空肠黏膜中。Enterokinase 的核心作用是激活消化酶原，可将胰蛋白酶原转化为胰蛋白酶。激活后的胰蛋白酶不仅能直接分解蛋白质，还能进一步激活其他酶原，如糜蛋白酶原、羧肽酶原和弹性蛋白酶原等，形成消化酶级联反应，促进蛋白质消化^[1][2][3]。

100 IU/μg Unit Definition: One unit is defined as the amount of enzyme needed to cleave 50 μg of fusion protein in 16 hours to 95% completion at 22°C in a buffer containing 25mM Tris-HCl, pH 8.0.

Bovine

P. pastoris

C-His

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Approximately 40 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against 20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 2 mM CaCl₂.

<0.2 EU/μg, determined by LAL method.

Reconstitute at 1 mg/mL in sterile deionied water, then dilute in sterile EK Storage Buffer (20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 2 mM CaCl₂).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Light A, et al. The amino-terminal sequence of the catalytic subunit of bovine enterokinase. J Protein Chem. 1991 Oct;10(5):475-80.  [Content Brief]

  [2]. Mann NS, et al. Enterokinase. Proc Soc Exp Biol Med. 1994 Jun;206(2):114-8.  [Content Brief]

  [3]. Kitamoto Y, et al. Enterokinase, the initiator of intestinal digestion, is a mosaic protease composed of a distinctive assortment of domains. Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7588-92.  [Content Brief]