1. 重组蛋白
  2. CAR-T related Proteins CD Antigens
  3. Epithelial Cell Adhesion Molecule (EpCAM) Stem Cell CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins
  4. CD326/EpCAM
  5. EpCAM/TROP1 蛋白, Human (HEK293, His)

EpCAM/TROP1 蛋白, Human (HEK293, His)

目录号: HY-P70154
COA 产品使用指南

EpCAM/TROP1 蛋白作为一种重要的均质相互作用分子,促进粘膜上皮中肠上皮细胞 (IEC) 和上皮内淋巴细胞 (IEL) 之间的直接接触。这一功能有助于建立抵抗粘膜感染的免疫屏障。EpCAM/TROP1 蛋白, Human (HEK293, His) 是重组的 EpCAM/TROP1 蛋白,由 HEK293 表达,带有 C-6*His 标签。EpCAM/TROP1 蛋白, Human (HEK293, His) 全长 242 个氨基酸,分子量约为 approximately 34-42 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The EpCAM/TROP1 protein serves as an important homogeneous interacting molecule that promotes direct contact between intestinal epithelial cells (IEC) and intraepithelial lymphocytes (IEL) in the mucosal epithelium. This feature helps establish an immune barrier against mucosal infections. EpCAM/TROP1 Protein, Human (HEK293, His) is the recombinant human-derived EpCAM/TROP1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of EpCAM/TROP1 Protein, Human (HEK293, His) is 242 a.a., with molecular weight of approximately 34-42 kDa.

研究背景

EpCAM/TROP1 蛋白作为一个关键实体出现,有可能充当物理同源相互作用分子,促进粘膜上皮的肠上皮细胞 (IEC) 和上皮内淋巴细胞 (IEL) 之间的直接接触,从而有助于建立免疫屏障作为抵抗粘膜感染的主要防御措施。除了在粘膜免疫中的作用之外,这种蛋白质在胚胎干细胞的增殖和分化中也发挥着重要作用。它还通过上调 FABP5、MYC 以及细胞周期蛋白 A 和 E 的表达来进一步表现出调节影响,表明 EpCAM/TROP1 参与关键细胞过程的调节。它的单体性质以及与磷酸化 CLDN7 的相互作用强调了所涉及的复杂的分子相互作用,为该蛋白质在细胞生理学中的多种功能提供了见解。

生物活性

Measured by the ability of the immobilized protein to support the adhesion of the L929 cells mouse fibroblast cell line. The ED50 for this effect is 0.7677 μg/mL, corresponding to a specific activity is 1.3×103 units/mg.

  • Measured by the ability of the immobilized protein to support the adhesion of the L929 cells mouse fibroblast cell line. The ED50 for this effect is 0.7677 μg/mL, corresponding to a specific activity is 1.3×103 units/mg.
种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

AAH14785.1 (Q24-K265)

基因 ID
蛋白结构
N-term
EpCAM (Q24-K265)
Accession # AAH14785.1
6*His
C-term
同用名
rHuEpithelial cell adhesion molecule/EpCAM, His; Epithelial Cell Adhesion Molecule; Ep-CAM; Adenocarcinoma-Associated Antigen; Cell Surface Glycoprotein Trop-1; Epithelial Cell Surface Antigen; Epithelial Glycoprotein; EGP; Epithelial Glycoprotein 314; EGP314; hEGP314; KSA; Tumor-Associated Calcium Signa
氨基酸序列

QEECVCENYKLAVNCFVNNNRQCQCTSVGAQNTVICSKLAAKCLVMKAEMNGSKLGRRAKPEGALQNNDGLYDPDCDESGLFKAKQCNGTSMCWCVNTAGVRRTDKDTEITCSERVRTYWIIIELKHKAREKPYDSKSLRTALQKEITTRYQLDPKFITSILYENNVITIDLVQNSSQKTQNDVDIADVAYYFEKDVKGESLFHSKKMDLTVNGEQLDLDPGQTLIYYVDEKAPEFSMQGLK

分子量

approximately 34-42 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2-7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EpCAM/TROP1 Protein, Human (HEK293, His)
目录号:
HY-P70154
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