1. 重组蛋白
  2. CAR-T related Proteins CD Antigens
  3. Epithelial Cell Adhesion Molecule (EpCAM) Stem Cell CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins
  4. CD326/EpCAM
  5. EpCAM/TROP1 蛋白, Rat (HEK293, His)

EpCAM/TROP1 蛋白, Rat (HEK293, His)

目录号: HY-P74172
产品使用指南

EpCAM/TROP1 蛋白作为一种多功能分子,促进粘膜上皮中肠上皮细胞 (IEC) 和上皮内淋巴细胞 (IEL) 之间的均匀相互作用。这表明在建立抵抗粘膜感染的免疫屏障方面发挥着至关重要的作用。EpCAM/TROP1 蛋白, Rat (HEK293, His) 是重组的 EpCAM/TROP1 蛋白,由 HEK293 表达,带有 C-His 标签。EpCAM/TROP1 蛋白, Rat (HEK293, His) 全长 243 个氨基酸,分子量约为 30-36 kDa。

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规格 价格 是否有货 数量
5 μg ¥600 In-stock
10 μg ¥950 In-stock
50 μg ¥2350 In-stock
100 μg ¥3800 In-stock
> 100 μg   询价  

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描述

The EpCAM/TROP1 protein serves as a multifunctional molecule that promotes homogeneous interactions between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) in the mucosal epithelium. This suggests a crucial role in establishing an immune barrier against mucosal infection. EpCAM/TROP1 Protein, Rat (HEK293, His) is the recombinant rat-derived EpCAM/TROP1 protein, expressed by HEK293 , with C-His labeled tag. The total length of EpCAM/TROP1 Protein, Rat (HEK293, His) is 243 a.a., with molecular weight of 30-36 kDa.

研究背景

EpCAM/TROP1 蛋白具有多方面的作用,可能充当物理同源相互作用分子,促进粘膜上皮的肠上皮细胞 (IEC) 和上皮内淋巴细胞 (IEL) 之间的直接接触。这种相互作用表明在建立免疫屏障方面具有关键功能,作为抵御粘膜感染的第一道防线。除了参与粘膜免疫之外,EpCAM/TROP1 在胚胎干细胞的增殖和分化中也发挥着重要作用。此外,它通过上调 FABP5、MYC 以及细胞周期蛋白 A 和 E 的表达来发挥调节作用,表明 EpCAM/TROP1 参与关键细胞过程的调节。它的单体性质以及与磷酸化 CLDN7 的相互作用强调了其分子相互作用的复杂性,揭示了其在细胞生理学中的多种功能。

生物活性

Measured by the ability of the immobilized protein to support the adhesion of the L929 mouse fibroblast cell line. The ED50 for this effect is 1.332 μg/mL in the presence of 0.5 μg/mL Fibronectin, corresponding to a specific activity is 750.751 units/mg.

种属

Rat

表达系统

HEK293

标签

C-6*His

蛋白编号

O55159 (Q24-T266)

基因 ID

171577  [NCBI]

蛋白结构
N-term
EpCAM (Q24-T266)
Accession # O55159
His
C-term
同用名
Epithelial cell adhesion molecule; Ep-CAM; EGP; KSA; CD326; TROP1
氨基酸序列

QKDCVCNNYKLTSRCYENENGECQCTSYGTQNTVICSKLASKCLVMKAEMTHSKSGRRMKPEGAIQNNDGLYDPECDEQGLFKAKQCNGTATCWCVNTAGVRRTDKDTEITCSERVRTYWIIIELKHKERAQPYNFESLHTALQDTFASRYMLNPKFIKSIMYENNVITIDLMQNSSQKTQDDVDIADVAYYFEKDVKGESLFHSSKSMDLRVNGELLDLDPGQTLIYYVDEKAPEFSMQGLT

分子量

Approximately 30-36 kDa due to the glycosylation.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
EpCAM/TROP1 Protein, Rat (HEK293, His)
目录号:
HY-P74172
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