EphA2 蛋白, Cynomolgus (HEK293, His-Avi)

EphA2 Protein, a receptor tyrosine kinase, engages in bidirectional signaling with ephrin-A ligands, influencing migration, adhesion, proliferation, and differentiation. EphA2 interacts with DSG1, inhibiting ERK1/ERK2 signaling, impacting cell adhesion and UV radiation-induced apoptosis. In development, EphA2 contributes to pattern formation, angiogenesis, hindbrain development, and mammary gland morphogenesis. Its interaction with Ephrin-A5 maintains lens transparency, and in bone remodeling, it regulates osteoclastogenesis and osteoblastogenesis through Ephrin-A2 interaction. EphA2 also acts as a receptor for hepatitis C virus in hepatocytes, facilitating viral entry and membrane fusion. EphA2 Protein, Cynomolgus (HEK293, His-Avi) is the recombinant cynomolgus-derived EphA2 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of EphA2 Protein, Cynomolgus (HEK293, His-Avi) is 511 a.a., with molecular weight of 60-70 kDa.

EphA2 蛋白是一种受体酪氨酸激酶，与邻近细胞上膜结合的肝配蛋白 A 配体相互作用，产生接触依赖性双向信号传导。该受体介导正向信号传导和反向信号传导途径。肝配蛋白-A1/EFNA1 的激活可调节多种细胞过程，包括迁移、整合素介导的粘附、增殖和分化。EphA2 还通过与 DSG1/desmoglein-1 相互作用以及抑制 ERK1/ERK2 信号通路来影响细胞粘附和分化。此外，它可能在紫外线辐射诱导的细胞凋亡中发挥作用，并独立于配体结合刺激趋化细胞迁移。在发育过程中，EphA2 参与模式形成并有助于胎儿组织的发育，例如血管生成、后脑发育和乳腺形态发生。它与肝配蛋白-A5/EFNA5 的相互作用对于通过调节晶状体纤维细胞的形状和相互作用来维持晶状体透明度至关重要。此外，EphA2 通过与 ephrin-A2/EFNA2 相互作用来调节破骨细胞生成和成骨细胞生成，从而参与骨重塑。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Cynomolgus

HEK293

C-Avi;C-His

Q1KL86 (A24-S534)

102146108  [NCBI]

60-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.