EphA3 蛋白, Human (HEK293, His)

The EphA3 protein is a receptor tyrosine kinase that promiscuously binds to membrane-bound ephrin ligands to initiate bidirectional signaling. Activation of EphA3 is known to preferentially bind to EFNA5 and regulates cell-cell adhesion, cytoskeletal organization, and migration. EphA3 Protein, Human (HEK293, His) is the recombinant human-derived EphA3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of EphA3 Protein, Human (HEK293, His) is 521 a.a., with molecular weight of ~67.0 kDa.

EphA3 蛋白是一种受体酪氨酸激酶，与相邻细胞上的膜结合肝配蛋白家族配体混杂结合，启动接触依赖性双向信号传导。源自受体的下游途径称为正向信号传导，而肝配蛋白配体下游的途径称为反向信号传导。EphA3 与肝配蛋白 A 配体高度混杂，对 EFNA5 表现出优先结合亲和力。被 EFNA5 激活后，EphA3 在调节细胞间粘附、细胞骨架组织和细胞迁移中发挥关键作用。此外，EphA3 参与心脏细胞迁移和分化，可能通过 EFNA1 激活来调节发育过程中房室管和房间隔的形成。在视网膜顶盖绘图中，EphA3 参与神经元的引导。此外，EphA3 可以控制神经肌肉回路发育过程中运动和感觉轴突的分离，但不是引导。

1.Measured by its binding ability in a functional ELISA. Immobilized EPHA3 at 0.2 μg/well can bind human EFNA5, the EC₅₀ of the protein is 0.4157-1.179 ng/mL.
2.Human EPHA3 protein his tag captured on COOH chip can bind Human EFNA5 protein Fc tag with an affinity constant of 13.8 nM as detected by LSPR Assay.

Human

HEK293

C-6*His

P29320 (E21-Q541)

2042  [NCBI]

ELIPQPSNEVNLLDSKTIQGELGWISYPSHGWEEISGVDEHYTPIRTYQVCNVMDHSQNNWLRTNWVPRNSAQKIYVELKFTLRDCNSIPLVLGTCKETFNLYYMESDDDHGVKFREHQFTKIDTIAADESFTQMDLGDRILKLNTEIREVGPVNKKGFYLAFQDVGACVALVSVRVYFKKCPFTVKNLAMFPDTVPMDSQSLVEVRGSCVNNSKEEDPPRMYCSTEGEWLVPIGKCSCNAGYEERGFMCQACRPGFYKALDGNMKCAKCPPHSSTQEDGSMNCRCENNYFRADKDPPSMACTRPPSSPRNVISNINETSVILDWSWPLDTGGRKDVTFNIICKKCGWNIKQCEPCSPNVRFLPRQFGLTNTTVTVTDLLAHTNYTFEIDAVNGVSELSSPPRQFAAVSITTNQAAPSPVLTIKKDRTSRNSISLSWQEPEHPNGIILDYEVKYYEKQEQETSYTILRARGTNVTISSLKPDTIYVFQIRARTAAGYGTNSRKFEFETSPDSFSISGESSQ

Approximately 67.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.