EphA4 蛋白, Rat (HEK293, His)

EphA4 Protein, a receptor tyrosine kinase, engages in contact-dependent bidirectional signaling with ephrin-A and ephrin-B ligands. Activated by GPI-anchored EFNA and transmembrane EFNB, EphA4 regulates cell morphology, integrin-dependent adhesion, and GTPase activity. Crucial in nervous system development, it guides axonal projections, segregates motor and sensory axons, and influences synaptic plasticity. In injury repair and angiogenesis, EphA4 inhibits axonal regeneration and contributes to vascular formation. Its promiscuity extends to thymic epithelium development, cochlear organ of Corti formation, and phosphorylation of CAPRIN1 for membraneless compartment formation. EphA4 Protein, Rat (HEK293, His) is the recombinant rat-derived EphA4 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphA4 Protein, Rat (HEK293, His) is 528 a.a., with molecular weight of ~70-80 kDa.

EphA4 是一种受体酪氨酸激酶，与相邻细胞上膜结合的肝配蛋白家族配体参与接触依赖性双向信号传导。EphA4 以其高度混杂性而著称，它独特地结合 GPI 锚定的肝配蛋白-A 和跨膜肝配蛋白-B 配体 (包括 EFNA1 和 EFNB3)，并在生理上被激活。被肝配蛋白配体激活后，EphA4 通过调节 Rac、Rap 和 Rho GTPases 活性来调节细胞形态和整合素依赖性细胞粘附。EphA4 在神经系统的发育中至关重要，它控制着轴突引导的各个步骤，包括皮质脊髓投射的建立以及神经肌肉回路发育过程中运动和感觉轴突的分离。在突触可塑性中，EphA4 通过在 “Tyr-15” 磷酸化 CDK5 来参与，从而调节 RHOA 和树突棘形态发生。此外，EphA4 通过防止轴突再生在损伤后修复中发挥作用，并在血管生成中发挥作用，有助于中枢神经系统血管形成。它的混杂性扩展了其对各种细胞间信号传导过程的参与，调节胸腺上皮的发育，并且在柯蒂耳蜗发育过程中，通过与 ADAM10 和 CADH1 形成三元复合物促进支柱细胞分离，从而导致 CADH1 的裂解和粘附连接的破坏。EphA4 还磷酸化 CAPRIN1，促进 CAPRIN1 依赖性无膜区室的形成^[1][2][3][4]。

Immobilized recombinant Rat EPHA4 Protein at 2 µg/mL (100 μL/well) can bind Recombinant rat EFNA5, The ED₅₀ for this effect is 35.43 ng/mL.

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  Immobilized recombinant Rat EPHA4 Protein at 2 µg/mL (100 μL/well) can bind Recombinant rat EFNA5, The ED₅₀ for this effect is 35.43 ng/mL.

Rat

HEK293

C-His

D3ZZK3 (V20-T547)

316539  [NCBI]

VTGSRVYPANEVTLLDSRSVQGELGWIASPLEGGWEEVSIMDEKNTPIRTYQVCNVMEASQNNWLRTDWITREGAQRVYIEIKFTLRDCNSLPGVMGTCKETFNLYYYESDNDKERFIRESQFGKIDTIAADESFTQVDIGDRIMKLNTEIRDVGPLSKKGFYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGADTSSLVEVRGSCVNNSEEKDVPKMYCGADGEWLVPIGNCLCNAGHEEQNGECQACKIGYYKALSTDATCAKCPPHSYSVWEGATSCTCDRGFFRADNDAASMPCTRPPSAPLNLISNVNETSVNLEWSSPQNTGGRQDISYNVVCKKCGAGDPSKCRPCGSGVHYTPQQNGLKTTRVSITDLLAHTNYTFEIWAVNGVSKYNPSPDQSVSVTVTTNQAAPSSIALVQAKEVTRYSVALAWLEPDRPNGVILEYEVKYYEKDQNERSYRIVRTAARNTDIKGLNPLTSYVFHVRARTAAGYGDFSEPLEVTTNTVPSRIIGDGANST

Approximately 70-80 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.