EphA7 蛋白, Rat (HEK293, His)

The EphA7 protein is a receptor tyrosine kinase that participates in bidirectional signaling with GPI-anchored ephrin A ligands (such as EFNA5). It affects brain development by regulating cell-cell adhesion and repulsion. EphA7 Protein, Rat (HEK293, His) is the recombinant rat-derived EphA7 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphA7 Protein, Rat (HEK293, His) is 512 a.a., with molecular weight of ~65-72 KDa.

EphA7 蛋白是一种受体酪氨酸激酶，可与邻近细胞上 GPI 锚定的肝配蛋白-A 配体相互作用，启动双向信号传导。这种接触依赖性信号传导，称为正向信号传导，发生在受体的下游，而反向信号传导发生在肝配蛋白配体的下游。在肝配蛋白-A 配体中，EFNA5 与 EphA7 特异性相互作用，通过调节细胞间粘附和排斥来影响大脑发育。EphA7 还在轴突引导中发挥作用，促进皮质丘脑和视网膜轴突的正确映射。此外，EphA7 可能通过依赖于 caspase (CASP3) 的促凋亡活性促进大脑发育。EphA7 的激活可导致 ERK 信号通路组件的磷酸化，包括 MAP2K1、MAP2K2、MAPK1 和 MAPK3。

Measured by its binding ability in a functional ELISA. When Recombinant Rat EphA7 is immobilized at 10 µg/mL (100 µL/well) can bind Biotinylated Recombinant mouse Ephrin-A4. The ED₅₀ for this effect is 212.3 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Rat EphA7 is immobilized at 10 µg/mL (100 µL/well) can bind Biotinylated Recombinant mouse Ephrin-A4. The ED₅₀ for this effect is 212.3ng/mL.

Rat

HEK293

C-His

P54759 (Q28-S539)

171287  [NCBI]

QAAKEVLLLDSKAQQTELEWISSPPSGWEEISGLDENYTPIRTYQVCQVMEPNQNNWLRTNWISKGNAQRIFVELKFTLRDCNSLPGVLGTCKETFNLYYYETDYDTGRNIRENLYVKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSKKGFYLAFQDVGACIALVSVKVYYKKCWSIIENLAVFPDTVTGSEFSSLVEVRGTCVSSAEEEAENSPRMHCSAEGEWLVPIGKCICKAGYQQKGDTCEPCGRRFYKSSSQDLQCSRCPTHSFSDREGSSRCECEDGYYRAPSDPPYVACTRPPSAPQNLIFNINQTTVSLEWSPPADNGGRNDVTYRILCKRCSWEQGECVPCGSNIGYMPQQTGLEDNYVTVMDLLAHANYTFEVEAVNGVSDLSRSQRLFAAVSITTGQAAPSQVSGVMKERVLQRSVELSWQEPEHPNGVITEYEIKYYEKDQRERTYSTLKTKSTSASINNLKPGTVYVFQIRAFTAAGYGNYSPRLDVATLEEAS

Approximately 65-72 kDa.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.