EphB1 蛋白, Human (HEK293, Fc)

The EphB1 protein is a receptor tyrosine kinase that promiscuously binds to the transmembrane ephrin-B ligand of adjacent cells, initiating bidirectional signaling. The forward signal originates from the receptor and the reverse signal originates from the ephrin ligand. EphB1 Protein, Human (HEK293, Fc) is the recombinant human-derived EphB1 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of EphB1 Protein, Human (HEK293, Fc) is 523 a.a., with molecular weight of 85-100 kDa.

EphB1 蛋白是一种受体酪氨酸激酶，与相邻细胞上的跨膜肝配蛋白 B 家族配体混杂结合，启动接触依赖性双向信号传导。源自受体的下游途径称为正向信号传导，而肝配蛋白配体下游的信号传导途径称为反向信号传导。该受体的同源/功能性肝配蛋白配体包括 EFNB1、EFNB2 和 EFNB3。在神经系统发育中，EphB1 通过在视交叉中线重定向同侧腹颞视网膜神经节细胞轴突（可能是通过与 EFNB2 的排斥相互作用）来调节视网膜轴突引导。在成人神经系统中，EphB1 与 EFNB3 一起控制海马神经祖细胞的趋化性、增殖和极性。除了在轴突引导中的作用之外，EphB1 与其他肝配蛋白 B 受体一起在树突棘的发育和成熟以及突触形成中发挥着至关重要的作用。此外，EphB1 可以调节血管生成，更一般地说，在靶细胞迁移和粘附中发挥作用。在被 EFNB1 和可能的其他肝配蛋白 B 配体激活后，EphB1 激活 MAPK/ERK 和 JNK 信号级联，分别调节细胞迁移和粘附。此外，EphB1 通过促进卫星细胞（肌肉干细胞）的自我更新并减少其激活和分化来参与维持卫星细胞库。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-hFc

P54762 (M18-P540)

2047  [NCBI]

MEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLP

85-100 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.