EphB3 蛋白, Rat (HEK293, His)

The EphB3 protein is a receptor tyrosine kinase that binds to the transmembrane ephrin B ligand to initiate bidirectional signaling. The forward signal originates from the receptor, while the reverse signal originates from the ephrin ligand. EphB3 Protein, Rat (HEK293, His) is the recombinant rat-derived EphB3 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphB3 Protein, Rat (HEK293, His) is 508 a.a., with molecular weight of ~60-70 kDa.

EphB3 蛋白是一种受体酪氨酸激酶，与相邻细胞上的跨膜肝配蛋白 B 家族配体混杂结合，启动接触依赖性双向信号传导。源自受体的下游途径称为正向信号传导，而肝配蛋白配体下游的信号传导途径称为反向信号传导。EphB3 通常表现出与 EPHB2 重叠和冗余的功能，特别是在发育过程中的轴突引导、调节形成胼胝体和前连合（大脑皮层颞叶之间的两个主要半球间连接）的神经元。除了在轴突引导中发挥作用外，EphB3 还与其他肝配蛋白 B 受体一起在树突棘的发育和成熟以及兴奋性突触的形成中发挥着至关重要的作用。该蛋白质还控制发育的各个方面，包括血管生成、上颚发育和胸腺上皮发育。通过 EFNB2/EPHB3 复合物内的正向和反向信号传导，EphB3 调节参与尿道管状化和泄殖腔分隔的细胞的迁移和粘附。值得注意的是，EphB3 通过将祖细胞与隐窝中的分化细胞分离，在肠上皮分化中发挥重要作用。

Immobilized mouse EPHB3 10 μg/mL (100 μL/well) can bind mouse EFNB1, The ED₅₀ for this effect is 8.187 ng/mL.

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  Immobilized mouse EPHB3 10 μg/mL (100 μL/well) can bind mouse EFNB1, The ED₅₀ for this effect is 8.187 ng/mL.

Rat

HEK293

C-His

D3ZH39 (L30-T537)

287989  [NCBI]

LEETLMDTKWVTSELAWTSHPESGWEEVSGYDEAMNPIRTYQVCNVRESSQNNWLRTGFIWRREVQRVYVELKFTVRDCNSIPNIPGSCKETFNLFYYEADSDVASASSPFWMENPYVKVDTIAPDESFSRLDAGRVNTKVRSFGPLSKAGFYLAFQDQGACMSLISVRAFYKKCASTTAGFALFPETLTGAEPTSLVIAPGTCIANAVEVSVPLKLYCNGDGEWMVPVGACTCATGHEPAAKETQCRACPPGSYKAKQGEGPCLPCPPNSRTTSPAASICTCHNNFYRADSDTADSACTTVPSPPRGVISNVNETSLILEWSEPRDLGGRDDLLYNVICKKCRGSSGAGGPATCSRCDDNVEFEPRQLGLTERRVHISHLLAHTRYTFEVQAVNGVSGKSPLPPRYAAVNITTNQAAPSEVPTLHLHSSSGSSLTLSWAPPERPNGVILDYEMKYFEKSKGIASTVTSQKNSVQLDGLQPDARYVVQVRARTVAGYGQYSRPAEFET

Approximately 60-70 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.