Ephrin-A1/EFNA1 蛋白, Mouse (HEK293, Fc-His)

The Ephrin-A1/EFNA1 protein is a GPI-binding ligand critical for migration, repulsion, and adhesion in developing neurons, blood vessels, and epithelia. It binds to nearby Eph receptors, initiating bidirectional signaling. Ephrin-A1/EFNA1 Protein, Mouse (HEK293, Fc-His) is the recombinant mouse-derived Ephrin-A1/EFNA1 protein, expressed by HEK293 , with C-hFc, C-6*His labeled tag. The total length of Ephrin-A1/EFNA1 Protein, Mouse (HEK293, Fc-His) is 164 a.a., with molecular weight of ~45-65 kDa.

Ephrin-A1/EFNA1 蛋白是 Eph 受体的细胞表面 GPI 结合配体，在神经元、血管和上皮发育过程中的迁移、排斥和粘附中发挥关键作用。它与相邻细胞上的 Eph 受体混杂结合，引发接触依赖性双向信号传导。EFNA1 诱导的 RAC1 GTPase 激活和血管内皮细胞迁移在血管生成和肿瘤新生血管形成中至关重要，这取决于磷酸化 EPHA2 募集 VAV2、VAV3 和 PI3 激酶 p85 亚基。值得注意的是，EFNA1 通过诱导酪氨酸磷酸化激活和下调 EPHA2，从而导致内化和降解，从而发挥抗癌作用。在神经胶质瘤中，它充当负调节因子，下调 EPHA2 和 FAK，从而发挥抑制肿瘤发生的作用。EFNA1可以诱导胚胎神经元生长锥的塌陷并调节树突棘形态发生。它以单体和同二聚体形式存在，与 EPHA2 形成异二聚体，并与一系列受体酪氨酸激酶结合，包括 EPHA1、EPHA3、EPHA4、EPHA5、EPHA6 和 EPHA7。

Mouse

HEK293

C-hFc;C-6*His

P52793 (D19-S182)

13636  [NCBI]

DRHIVFWNSSNPKFREEDYTVHVQLNDYLDIICPHYEDDSVADAAMERYTLYMVEHQEYVACQPQSKDQVRWNCNRPSAKHGPEKLSEKFQRFTPFILGKEFKEGHSYYYISKPIYHQESQCLKLKVTVNGKITHNPQAHVNPQEKRLQADDPEVQVLHSIGYS

Approximately 45-65 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.